{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:56:03Z","timestamp":1791280563384,"version":"4.1.0"},"reference-count":34,"publisher":"American Physiological Society","issue":"4","funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft (DFG)","doi-asserted-by":"publisher","award":["KO 4877\/ 2-1"],"award-info":[{"award-number":["KO 4877\/ 2-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"PATE Program UNiversity of T\u00fcbingen","award":["2296-0-0"],"award-info":[{"award-number":["2296-0-0"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2019,4,1]]},"abstract":"<jats:p>Doxapram is a respiratory stimulant used for decades as a treatment option in apnea of prematurity refractory to methylxanthine treatment. Its mode of action, however, is still poorly understood. We investigated direct effects of doxapram on the pre-B\u00f6tzinger complex (PreB\u00f6tC) and on a downstream motor output system, the hypoglossal nucleus (XII), in the transverse brainstem slice preparation. While doxapram has only a modest stimulatory effect on frequency of activity generated within the PreB\u00f6tC, a much more robust increase in the amplitude of population activity in the subsequent motor output generated in the XII was observed. In whole cell patch-clamp recordings of PreB\u00f6tC and XII neurons, we confirmed significantly increased firing of evoked action potentials in XII neurons in the presence of doxapram, while PreB\u00f6tC neurons showed no significant alteration in firing properties. Interestingly, the amplitude of activity in the motor output was not increased in the presence of doxapram compared with control conditions during hypoxia. We conclude that part of the stimulatory effects of doxapram is caused by direct input on brainstem centers with differential effects on the rhythm generating kernel (PreB\u00f6tC) and the downstream motor output (XII).<\/jats:p>\n                  <jats:p>NEW &amp; NOTEWORTHY The clinically used respiratory stimulant doxapram has distinct effects on the rhythm generating kernel (pre-B\u00f6tzinger complex) and motor output centers (nucleus hypoglossus). These effects are obliterated during hypoxia and are mediated by distinct changes in the intrinsic properties of neurons of the nucleus hypoglossus and synaptic transmission received by pre-B\u00f6tzinger complex neurons.<\/jats:p>","DOI":"10.1152\/jn.00304.2018","type":"journal-article","created":{"date-parts":[[2019,1,30]],"date-time":"2019-01-30T14:25:01Z","timestamp":1548858301000},"page":"1102-1110","source":"Crossref","is-referenced-by-count":12,"title":["Doxapram stimulates respiratory activity through distinct activation of neurons in the nucleus hypoglossus and the pre-B\u00f6tzinger complex"],"prefix":"10.1152","volume":"121","author":[{"given":"Sandra","family":"Kruszynski","sequence":"first","affiliation":[{"name":"Department of Neonatology, T\u00fcbingen University Hospital, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kornelijus","family":"Stanaitis","sequence":"additional","affiliation":[{"name":"Department of Neurology and 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