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I<jats:sub>h<\/jats:sub> current density was larger in P25\u201328 than P7\u201310 rats, increasing 410% for small cells (&lt;30 pF) and 200% for larger cells (&gt;30 pF). The half-maximum activation voltage ( V<jats:sub>1\/2<\/jats:sub>) of I<jats:sub>h<\/jats:sub> was \u2212102 mV in P7\u201310 rats and in P25\u201328 rats shifted 7 mV toward positive voltages. At both ages, intracellular cAMP increased I<jats:sub>h<\/jats:sub> current density, decreased its activation time constant (\u03c4), and resulted in a rightward shift of V<jats:sub>1\/2<\/jats:sub> by 9 mV. Perfusion of 8-BrcAMP increased I<jats:sub>h<\/jats:sub> amplitude and speed up its activation kinetics. I<jats:sub>h<\/jats:sub> was blocked by Cs<jats:sup>+<\/jats:sup>, zatebradine, and ZD7288. As expected, these drugs also reduced the voltage sag caused with hyperpolarizing pulses and prevented the postpulse action potential generation without changes in the resting potential. RT-PCR analysis showed that HCN1 and HCN2 subunits were predominantly amplified in vestibular ganglia and end organs and HCN3 and HCN4 to a lesser extent. Immunohistochemistry showed that the four HCN subunits were differentially expressed (HCN1 &gt; HCN2 &gt; HCN3 \u2265 HCN4) in ganglion slices and in cultured neurons at both P7\u201310 and P25\u201328 stages. Developmental changes shifted V<jats:sub>1\/2<\/jats:sub> of I<jats:sub>h<\/jats:sub> closer to the resting membrane potential, increasing its functional role. 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