{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T22:29:26Z","timestamp":1784759366893,"version":"3.55.0"},"reference-count":111,"publisher":"American Physiological Society","issue":"5","funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2011616"],"award-info":[{"award-number":["2011616"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["ACI-1548562"],"award-info":[{"award-number":["ACI-1548562"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["ACI-1928147"],"award-info":[{"award-number":["ACI-1928147"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2023,5,1]]},"abstract":"<jats:p> We used a computational model of the active zone (AZ) in the mammalian neuromuscular junction to investigate Lambert-Eaton myasthenic syndrome (LEMS) pathophysiology. This model suggests that disruptions in presynaptic active zone organization and protein content (particularly synaptotagmin), beyond the simple removal of presynaptic calcium channels, play an important role in LEMS pathophysiology. <\/jats:p>","DOI":"10.1152\/jn.00404.2022","type":"journal-article","created":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T12:07:05Z","timestamp":1681906025000},"page":"1259-1277","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Simulations of active zone structure and function at mammalian NMJs predict that loss of calcium channels alone is not sufficient to replicate LEMS effects"],"prefix":"10.1152","volume":"129","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8820-606X","authenticated-orcid":false,"given":"Scott P.","family":"Ginebaugh","sequence":"first","affiliation":[{"name":"Department of Neuroscience, Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0604-0516","authenticated-orcid":false,"given":"Yomna","family":"Badawi","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9434-758X","authenticated-orcid":false,"given":"Rozita","family":"Laghaei","sequence":"additional","affiliation":[{"name":"Biomedical Application Group, Pittsburgh Supercomputing Center, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenn","family":"Mersky","sequence":"additional","affiliation":[{"name":"Biomedical Application Group, Pittsburgh Supercomputing Center, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caleb J.","family":"Wallace","sequence":"additional","affiliation":[{"name":"Biomedical Application Group, Pittsburgh Supercomputing Center, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5821-2207","authenticated-orcid":false,"given":"Tyler B.","family":"Tarr","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassandra","family":"Kaufhold","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Reddel","sequence":"additional","affiliation":[{"name":"Department of Clinical Neurology, Concord Hospital, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2005-9726","authenticated-orcid":false,"given":"Stephen D.","family":"Meriney","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20672"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4629-12.2013"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/s0165-5728(00)00431-8"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2008.04.040"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncl.2018.01.008"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199506013322203"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/s0022-510x(96)05303-8"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000037485.56217.5f"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510x(95)00162-u"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3519-06.2006"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601103"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-200004000-00002"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410350112"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2014.08.007"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2013.05.023"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00198.2014"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00168.2017"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1981.sp013586"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.24.7636"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1984.tb02733.x"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410220203"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020865"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1530\/REP-09-0454"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1998.80.3.1056"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.021048"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.276493"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)76153-5"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000008747"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-020-03282-x"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.5535\/arm.2011.35.5.658"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(91)82344-X"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1137\/070692017"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.12.5747"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2415-19.2020"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2024029118"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.02.047"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.3.270"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e05-04-0277"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2010.02.041"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.81.2.275"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1023\/a:1006995010453"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21975"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(87)90027-3"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.170092"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.100302"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021665"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp017248"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(02)73907-6"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(04)74324-6"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1007\/s10827-008-0079-5"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(97)78091-3"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1038\/75737"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb1310"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1126\/science.1142614"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1038\/nature16507"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)37523-3"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.22.13995"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.343418"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.5.1125"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2019.01.035"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0228348"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1093\/biomet\/57.1.97"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1063\/1.1699114"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2006.04282.x"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00708.2009"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0437991100"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1007\/BF00581322"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1989.tb12584.x"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1990.tb12061.x"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1080\/0968768021000035087"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.284133"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.04057"},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.12.026"},{"key":"B74","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2753-14.2015"},{"key":"B75","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.11.019"},{"key":"B76","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2016.01.005"},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1983.sp014943"},{"key":"B78","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb11284.x"},{"key":"B79","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.262787699"},{"key":"B80","doi-asserted-by":"publisher","DOI":"10.1023\/a:1021985521748"},{"key":"B81","doi-asserted-by":"publisher","DOI":"10.1023\/b:jobb.0000008033.02320.10"},{"key":"B82","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1089-05.2005"},{"key":"B83","doi-asserted-by":"publisher","DOI":"10.1177\/1073858405281898"},{"key":"B84","doi-asserted-by":"publisher","DOI":"10.1212\/wnl.50.2.470"},{"key":"B85","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.54.5.452"},{"key":"B86","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinph.2004.12.013"},{"key":"B87","doi-asserted-by":"publisher","DOI":"10.3988\/jcn.2012.8.4.305"},{"key":"B88","doi-asserted-by":"publisher","DOI":"10.1002\/jgf2.66"},{"key":"B89","doi-asserted-by":"publisher","DOI":"10.1358\/dot.2020.56.10.3137144"},{"key":"B90","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2018.21321"},{"key":"B91","doi-asserted-by":"publisher","DOI":"10.1177\/1060028019864574"},{"key":"B92","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1998.tb11022.x"},{"key":"B93","doi-asserted-by":"publisher","DOI":"10.1002\/mus.26052"},{"key":"B94","doi-asserted-by":"publisher","DOI":"10.1097\/CND.0000000000000239"},{"key":"B95","doi-asserted-by":"publisher","DOI":"10.1212\/CPJ.0000000000000321"},{"key":"B96","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.70.2.212"},{"key":"B97","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(11)70245-9"},{"key":"B98","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000002185"},{"key":"B99","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.2009.35"},{"key":"B100","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(92)90408-2"},{"key":"B101","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-017-8627-4"},{"key":"B102","doi-asserted-by":"publisher","DOI":"10.1080\/00207454.2016.1207644"},{"key":"B103","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-018-8959-8"},{"key":"B104","doi-asserted-by":"publisher","DOI":"10.1016\/s0022-510x(99)00125-2"},{"key":"B105","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1998.tb10994.x"},{"key":"B106","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.06.012"},{"key":"B107","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3338"},{"key":"B108","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3771-10.2011"},{"key":"B109","doi-asserted-by":"publisher","DOI":"10.1038\/nature03112"},{"key":"B110","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2016.08.008"},{"key":"B111","doi-asserted-by":"publisher","DOI":"10.1016\/s0168-0102(99)00135-2"}],"container-title":["Journal of Neurophysiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/jn.00404.2022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T13:24:35Z","timestamp":1684329875000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/jn.00404.2022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,1]]},"references-count":111,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5,1]]}},"alternative-id":["10.1152\/jn.00404.2022"],"URL":"https:\/\/doi.org\/10.1152\/jn.00404.2022","relation":{},"ISSN":["0022-3077","1522-1598"],"issn-type":[{"value":"0022-3077","type":"print"},{"value":"1522-1598","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,1]]},"assertion":[{"value":"2022-09-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-14","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}