{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T14:34:19Z","timestamp":1781620459671,"version":"3.54.5"},"reference-count":51,"publisher":"American Physiological Society","issue":"2","funder":[{"name":"Auditory Neuroscience Training Grant","award":["DC005361"],"award-info":[{"award-number":["DC005361"]}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft (DFG)","doi-asserted-by":"publisher","award":["SFB870\/2-A10"],"award-info":[{"award-number":["SFB870\/2-A10"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council (MRC)","doi-asserted-by":"publisher","award":["K005170"],"award-info":[{"award-number":["K005170"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health (NIH)","doi-asserted-by":"publisher","award":["P30Core DC04661"],"award-info":[{"award-number":["P30Core DC04661"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health (NIH)","doi-asserted-by":"publisher","award":["R01 DC03829"],"award-info":[{"award-number":["R01 DC03829"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health (NIH)","doi-asserted-by":"publisher","award":["RO1 DC02739"],"award-info":[{"award-number":["RO1 DC02739"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2017,2,1]]},"abstract":"<jats:p> The medial nucleus of the trapezoid body (MNTB) is an important source of inhibition during the computation of sound location. It transmits fast and precisely timed action potentials at high frequencies; this requires an efficient calcium clearance mechanism, in which plasma membrane calcium ATPase 2 (PMCA2) is a key component. Deafwaddler ( dfw<jats:sup> 2J<\/jats:sup>) mutant mice have a null mutation in PMCA2 causing deafness in homozygotes ( dfw<jats:sup> 2J<\/jats:sup>\/ dfw<jats:sup> 2J<\/jats:sup>) and high-frequency hearing loss in heterozygotes (+\/ dfw<jats:sup> 2J<\/jats:sup>). Despite the deafness phenotype, no significant differences in MNTB volume or cell number were observed in dfw<jats:sup> 2J<\/jats:sup> homozygous mutants, suggesting that PMCA2 is not required for MNTB neuron survival. The MNTB tonotopic axis encodes high to low sound frequencies across the medial to lateral dimension. We discovered a cell size gradient along this axis: lateral neuronal somata are significantly larger than medially located somata. This size gradient is decreased in +\/ dfw<jats:sup> 2J<\/jats:sup> and absent in dfw<jats:sup> 2J<\/jats:sup>\/ dfw<jats:sup> 2J<\/jats:sup>. The lack of acoustically driven input suggests that sound-evoked activity is required for maintenance of the cell size gradient. This hypothesis was corroborated by selective elimination of auditory hair cell activity with either hair cell elimination in Pou4f3 DTR mice or inner ear tetrodotoxin (TTX) treatment. The change in soma size was reversible and recovered within 7 days of TTX treatment, suggesting that regulation of the gradient is dependent on synaptic activity and that these changes are plastic rather than permanent. <\/jats:p><jats:p> NEW &amp; NOTEWORTHY Neurons of the medial nucleus of the trapezoid body (MNTB) act as fast-spiking inhibitory interneurons within the auditory brain stem. The MNTB is topographically organized, with low sound frequencies encoded laterally and high frequencies medially. We discovered a cell size gradient along this axis: lateral neurons are larger than medial neurons. The absence of this gradient in deaf mice lacking plasma membrane calcium ATPase 2 suggests an activity-dependent, calcium-mediated mechanism that controls neuronal soma size. <\/jats:p>","DOI":"10.1152\/jn.00528.2016","type":"journal-article","created":{"date-parts":[[2016,11,23]],"date-time":"2016-11-23T22:30:52Z","timestamp":1479940252000},"page":"756-766","source":"Crossref","is-referenced-by-count":21,"title":["Maintenance of neuronal size gradient in MNTB requires sound-evoked activity"],"prefix":"10.1152","volume":"117","author":[{"given":"Jessica H.","family":"Weatherstone","sequence":"first","affiliation":[{"name":"Virginia Merrill Bloedel Hearing Research Center, Department of Otolaryngology-Head and Neck Surgery, and Department of Pharmacology, University of Washington School of Medicine, Seattle, Washington;"},{"name":"Virginia Merrill Bloedel Hearing Research Center, Department of Otolaryngology-Head and Neck Surgery, and Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Conny","family":"Kopp-Scheinpflug","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Psychology and Behaviour, University of Leicester, Leicester, United Kingdom;"},{"name":"Division of Neurobiology, Department Biology II, Ludwig-Maximilians University Munich, Planegg-Martinsried, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Pilati","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Psychology and Behaviour, University of Leicester, Leicester, United Kingdom;"},{"name":"Autifony Srl Laboratories, Medicines Research Centre, Verona, Italy; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Virginia Merrill Bloedel Hearing Research Center, Department of Otolaryngology-Head and Neck Surgery, and Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington;"},{"name":"Department of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian D.","family":"Forsythe","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Psychology and Behaviour, University of Leicester, Leicester, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin W.","family":"Rubel","sequence":"additional","affiliation":[{"name":"Virginia Merrill Bloedel Hearing Research Center, Department of Otolaryngology-Head and Neck Surgery, and Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce L.","family":"Tempel","sequence":"additional","affiliation":[{"name":"Virginia Merrill Bloedel Hearing Research Center, Department of Otolaryngology-Head and Neck Surgery, and Department of Pharmacology, University of Washington School of Medicine, Seattle, Washington;"},{"name":"Virginia Merrill Bloedel Hearing Research Center, Department of Otolaryngology-Head and Neck Surgery, and Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00457-7"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-200110000-00001"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-14-05840.2002"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5481.953"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp021095"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1042\/BST0350913"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.14.030191.001505"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-14-05066.2001"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2004.03604.x"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00783.2006"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0609775104"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22576"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00601.2014"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22393"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1709-12.2012"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(90)90111-2"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.172387"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1007\/s10162-002-2010-5"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2008.01.088"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(01)00420-8"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.30.18693"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.098780"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-03-01486.1994"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3285-09.2009"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5054-12.2013"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2006.11.006"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2004.05.003"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4378-09.2010"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903480106"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902830403"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1177\/019459989110400103"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(01)00356-2"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-66880-7_5"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-006-0272-7"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa043899"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2013.103"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901860207"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1377-09.2009"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1038\/1284"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1102"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-24-09162.2000"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(87)90087-6"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1177\/1073858403252228"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5043-12.2013"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2179-14.2015"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2604"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1038\/nrn705"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4554-03.2004"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.183bo.x"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22045"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-02-00610.1998"}],"container-title":["Journal of Neurophysiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jn.00528.2016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T02:25:16Z","timestamp":1567995916000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jn.00528.2016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,1]]},"references-count":51,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,2,1]]}},"alternative-id":["10.1152\/jn.00528.2016"],"URL":"https:\/\/doi.org\/10.1152\/jn.00528.2016","relation":{},"ISSN":["0022-3077","1522-1598"],"issn-type":[{"value":"0022-3077","type":"print"},{"value":"1522-1598","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,1]]}}}