{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:15:46Z","timestamp":1791112546713,"version":"4.1.0"},"reference-count":37,"publisher":"American Physiological Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2012,12,1]]},"abstract":"<jats:p> During development, synaptic exocytosis by cochlear hair cells is first initiated by patterned spontaneous Ca<jats:sup>2+<\/jats:sup> spikes and, at the onset of hearing, by sound-driven graded depolarizing potentials. The molecular reorganization occurring in the hair cell synaptic machinery during this developmental transition still remains elusive. We characterized the changes in biophysical properties of voltage-gated Ca<jats:sup>2+<\/jats:sup> currents and exocytosis in developing auditory hair cells of a precocial animal, the domestic chick. We found that immature chick hair cells (embryonic days 10\u201312) use two types of Ca<jats:sup>2+<\/jats:sup> currents to control exocytosis: low-voltage-activating, rapidly inactivating (mibefradil sensitive) T-type Ca<jats:sup>2+<\/jats:sup> currents and high-voltage-activating, noninactivating (nifedipine sensitive) L-type currents. Exocytosis evoked by T-type Ca<jats:sup>2+<\/jats:sup> current displayed a fast release component (RRP) but lacked the slow sustained release component (SRP), suggesting an inefficient recruitment of distant synaptic vesicles by this transient Ca<jats:sup>2+<\/jats:sup> current. With maturation, the participation of L-type Ca<jats:sup>2+<\/jats:sup> currents to exocytosis largely increased, inducing a highly Ca<jats:sup>2+<\/jats:sup> efficient recruitment of an RRP and an SRP component. Notably, L-type-driven exocytosis in immature hair cells displayed higher Ca<jats:sup>2+<\/jats:sup> efficiency when triggered by prerecorded native action potentials than by voltage steps, whereas similar efficiency for both protocols was found in mature hair cells. This difference likely reflects a tighter coupling between release sites and Ca<jats:sup>2+<\/jats:sup> channels in mature hair cells. Overall, our results suggest that the temporal characteristics of Ca<jats:sup>2+<\/jats:sup> entry through T-type and L-type Ca<jats:sup>2+<\/jats:sup> channels greatly influence synaptic release by hair cells during cochlear development. <\/jats:p>","DOI":"10.1152\/jn.00555.2012","type":"journal-article","created":{"date-parts":[[2012,9,13]],"date-time":"2012-09-13T06:13:35Z","timestamp":1347516815000},"page":"3116-3123","source":"Crossref","is-referenced-by-count":13,"title":["The temporal characteristics of Ca<sup>2+<\/sup> entry through L-type and T-type Ca<sup>2+<\/sup> channels shape exocytosis efficiency in chick auditory hair cells during development"],"prefix":"10.1152","volume":"108","author":[{"given":"Snezana","family":"Levic","sequence":"first","affiliation":[{"name":"Equipe Neurophysiologie de la Synapse Auditive, Unit\u00e9 Mixte de Recherche, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U587 et Universit\u00e9 Bordeaux Segalen, Institut des 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