{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:03:50Z","timestamp":1760954630843},"reference-count":37,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:p> Functional interactions between ligand-gated, voltage-, and Ca<jats:sup>2+<\/jats:sup>-activated ion channels are essential to the properties of excitable cells and thus to the working of the nervous system. The outer hair cells in the mammalian cochlea receive efferent inputs from the brain stem through cholinergic nerve fibers that form synapses at their base. The acetylcholine released from these efferent fibers activates fast inhibitory postsynaptic currents mediated, to some extent, by small-conductance Ca<jats:sup>2+<\/jats:sup>-activated K<jats:sup>+<\/jats:sup> channels (SK) that had not been cloned. Here we report the cloning, characterization, and expression of a complete SK2 cDNA from the mouse cochlea. The cDNAs of the mouse cochlea \u03b19 and \u03b110 acetylcholine receptors were also obtained, sequenced, and coexpressed with the SK2 channels. Human cultured cell lines transfected with SK2 yielded Ca<jats:sup>2+<\/jats:sup>-sensitive K<jats:sup>+<\/jats:sup> current that was blocked by dequalinium chloride and apamin, known blockers of SK channels. Xenopus oocytes injected with SK2 in vitro transcribed RNA, under conditions where only outward K<jats:sup>+<\/jats:sup> currents could be recorded, expressed an outward current that was sensitive to EGTA, dequalinium chloride, and apamin. In HEK-293 cells cotransfected with cochlear SK2 plus \u03b19\/\u03b110 receptors, acetylcholine induced an inward current followed by a robust outward current. The results indicate that SK2 and the \u03b19\/\u03b110 acetylcholine receptors are sufficient to partly recapitulate the native hair cell efferent synaptic response. <\/jats:p>","DOI":"10.1152\/jn.00630.2003","type":"journal-article","created":{"date-parts":[[2004,3,8]],"date-time":"2004-03-08T20:38:59Z","timestamp":1078778339000},"page":"1536-1544","source":"Crossref","is-referenced-by-count":34,"title":["Cloning and Expression of a Small-Conductance Ca<sup>2+<\/sup>-Activated K<sup>+<\/sup> Channel From the Mouse Cochlea: Coexpression with \u03b19\/\u03b110 Acetylcholine Receptors"],"prefix":"10.1152","volume":"91","author":[{"given":"Liping","family":"Nie","sequence":"first","affiliation":[{"name":"Department of Otolaryngology, University of California, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haitao","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, University of California, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei-Fang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of California, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nipavan","family":"Chiamvimonvat","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine, University of California, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirk W.","family":"Beisel","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Creighton University, Omaha, Nebraska 68178"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ebenezer N.","family":"Yamoah","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, University of California, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana E.","family":"V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, University of California, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"REF1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(99)00078-9"},{"key":"REF2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-08-02574.1996"},{"key":"REF3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.26.12725"},{"key":"REF4","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.78.5.936"},{"key":"REF5","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.1998.00098.x"},{"key":"REF6","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90555-X"},{"key":"REF7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.051622798"},{"key":"REF8","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.12-03-00800.1992"},{"key":"REF9","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1992.0039"},{"key":"REF10","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(83)90025-8"},{"key":"REF11","doi-asserted-by":"publisher","DOI":"10.1007\/BF00656997"},{"key":"REF12","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)01947-8"},{"key":"REF13","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1991.0065"},{"key":"REF14","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.37.23195"},{"key":"REF15","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-20-08830.1999"},{"key":"REF16","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5282.1709"},{"key":"REF17","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.18.7057"},{"key":"REF18","unstructured":"Luebke A. 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