{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T11:09:28Z","timestamp":1778843368723,"version":"3.51.4"},"reference-count":57,"publisher":"American Physiological Society","issue":"4","funder":[{"DOI":"10.13039\/100000055","name":"HHS | NIH | National Institute on Deafness and Other Communication Disorders (NIDCD)","doi-asserted-by":"publisher","award":["R01 DC 008855"],"award-info":[{"award-number":["R01 DC 008855"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"HHS | NIH | National Institute on Aging (U.S. National Institute on Aging)","doi-asserted-by":"publisher","award":["R21 AG 053690"],"award-info":[{"award-number":["R21 AG 053690"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000026","name":"HHS | NIH | National Institute on Drug Abuse (NIDA)","doi-asserted-by":"publisher","award":["P30 DA 015663"],"award-info":[{"award-number":["P30 DA 015663"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2018,10,1]]},"abstract":"<jats:p> Nicotinic acetylcholine receptors (nAChRs) regulate information transfer across the main olfactory bulb by instituting a high-pass intensity filter allowing for the filtering out of weak inputs. Excitation-driven inhibition of the glomerular microcircuit via GABA release from periglomerular cells appears to underlie this effect of nAChR activation. The multiplicity of nAChR subtypes and cellular locations raises questions about their respective roles in mediating their effects on the glomerular output. In this study, we address this issue by targeting heteromeric nAChRs using receptor knockouts (KOs) for the two dominant nAChR \u03b2-subunit genes known to be expressed in the central nervous system. KOs of the \u03b2<jats:sub>2<\/jats:sub>-nAChR subunit did not affect nAChR currents from mitral cells (MCs) but attenuated those from the external tufted (ET) cells. In slices from these animals, activation of nAChRs still effectively inhibited excitatory postsynaptic currents (EPSCs) and firing on MCs evoked by the olfactory nerve (ON) stimulation, thereby indicating that the filter mechanism was intact. On the other hand, recordings from \u03b2<jats:sub>4<\/jats:sub>-KOs showed that nAChR responses from MCs were abolished and those from ET cells were attenuated. Excitation-driven feedback was abolished as was the effect of nAChR activation on ON-evoked EPSCs. Experiments using calcium imaging showed that one possible consequence of the \u03b2<jats:sub>2<\/jats:sub>-subunit activation might be to alter the time course of calcium transients in juxtaglomerular neurons suggesting a role for these receptors in calcium signaling. Our results indicate that nAChRs containing the \u03b2<jats:sub>4<\/jats:sub>-subunit are critical in the filtering of odor inputs and play a determinant role in the cholinergic modulation of glomerular output. <\/jats:p><jats:p> NEW &amp; NOTEWORTHY In this study, using receptor gene knockouts we examine the relative contributions of heteromeric nAChR subtypes located on different cell types to this effect of receptor activation. Our results demonstrate that nAChRs containing the \u03b2<jats:sub>4<\/jats:sub>-subunit activate MCs resulting in feedback inhibition from glomerular interneurons. This period of inhibition results in the selective filtering of weak odor inputs providing one mechanism by which nAChRs can enhance discrimination between two closely related odors. <\/jats:p>","DOI":"10.1152\/jn.00925.2017","type":"journal-article","created":{"date-parts":[[2018,8,8]],"date-time":"2018-08-08T13:16:05Z","timestamp":1533734165000},"page":"2036-2048","source":"Crossref","is-referenced-by-count":5,"title":["A dominant role for the beta 4 nicotinic receptor subunit in nicotinic modulation of glomerular microcircuits in the mouse olfactory bulb"],"prefix":"10.1152","volume":"120","author":[{"given":"Michael S.","family":"Spindle","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics and the Neuroscience Program, University of Colorado, School of Medicine, Aurora, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pirooz V.","family":"Parsa","sequence":"additional","affiliation":[{"name":"Department of Physiology and 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