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Very broad action potentials (resulting from TEA application) decreased prolonged depolarization amplitude, indicating that strong Ca<jats:sup>2+<\/jats:sup>influx did not necessarily promote the response. The prolonged depolarization current ( I<jats:sub>PD<\/jats:sub>) was recorded after 5-Hz, 10-s trains of square voltage pulses of varying duration (10\u2013150 ms). Despite Ca<jats:sup>2+<\/jats:sup>influx increasing steadily with pulse duration, I<jats:sub>PD<\/jats:sub>was most reliably initiated at 100 ms, suggesting a Ca<jats:sup>2+<\/jats:sup>window or limit exists for triggering I<jats:sub>PD<\/jats:sub>. Consistent with this, modestly broader action potentials, evoked by lengthening the train current-pulse duration, resulted in smaller prolonged depolarizations. With respect to the properties of I<jats:sub>PD<\/jats:sub>, it displayed a linear current\u2013voltage relationship with a reversal potential of about \u221245 mV that was shifted to approximately \u221225 mV by lowering internal K<jats:sup>+<\/jats:sup>or about \u221256 mV by lowering external Na<jats:sup>+<\/jats:sup>and Ca<jats:sup>2+<\/jats:sup>. I<jats:sub>PD<\/jats:sub>was blocked by Gd<jats:sup>3+<\/jats:sup>, but was not antagonized by MDL-123302A, SKF-96365, 2-APB, tetrodotoxin, or flufenamic acid. 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