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To provide a fundamental understanding of the properties of GABAergic neurotransmission in mammalian cochlear nucleus, we investigated the reversal potential of the GABA-evoked currents ( E<jats:sub>GABA<\/jats:sub>) by means of gramicidin-perforated-patch recordings in developing SBC. The action of GABA switches from depolarizing to hyperpolarizing by the postnatal day 7 due to the negative shift in E<jats:sub>GABA<\/jats:sub>. Furthermore, we studied the expression pattern of the K<jats:sup>+<\/jats:sup>-Cl<jats:sup>\u2212<\/jats:sup>-extruding cotransporter KCC2, previously shown to induce a switch from neonatal Cl<jats:sup>\u2212<\/jats:sup> efflux to the mature Cl<jats:sup>\u2212<\/jats:sup> influx in various neuron types, thereby causing a shift from depolarizing to hyperpolarizing GABA action. The KCC2 protein is expressed in SBC already at birth, yet its activity is attained toward the end of the first postnatal week as indicated by pharmacological inhibition. Interruption of the Cl<jats:sup>\u2212<\/jats:sup> extrusion by [(dihydroindenyl)oxy] alkanoic acid or furosemide gradually shifted E<jats:sub>GABA<\/jats:sub> in positive direction with increasing maturity, suggesting that KCC2 could be involved in maintaining low [Cl<jats:sup>\u2212<\/jats:sup>]<jats:sub>i<\/jats:sub> after the postnatal day 7 thereby providing the hyperpolarizing Cl<jats:sup>\u2212<\/jats:sup>-mediated inhibition in SBC. <\/jats:p>","DOI":"10.1152\/jn.01150.2006","type":"journal-article","created":{"date-parts":[[2007,6,28]],"date-time":"2007-06-28T00:44:31Z","timestamp":1182991471000},"page":"1634-1644","source":"Crossref","is-referenced-by-count":27,"title":["Development of Chloride-Mediated Inhibition in Neurons of the Anteroventral Cochlear Nucleus of Gerbil (<i>Meriones unguiculatus<\/i>)"],"prefix":"10.1152","volume":"98","author":[{"given":"Ivan","family":"Milenkovi\u0107","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirko","family":"Witte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rostislav","family":"Ture\u010dek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Heinrich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Reinert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudolf","family":"R\u00fcbsamen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","unstructured":"Altschuler RA, Juiz JM, Shore SE, Bledsoe SC, Helfert RH, Wenthold RJ. 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