{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:28:45Z","timestamp":1761514125890},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1991,8,1]]},"abstract":"<jats:p> 1. In this electrophysiological study, action potentials from single C-fibers were recorded in fine filaments teased from the rat saphenous nerve. We evaluated the effect of pharmacological agents on the responses of C-fiber mechanoheat nociceptors (C-MH; n = 53) after sustained suprathreshold and subthreshold stimuli. 2. Sustained suprathreshold mechanical stimuli elicit an immediate burst of activity that quickly adapts to a low-level firing that is maintained during the stimulus. Sustained subthreshold stimuli activate C-MHs after a delay and elicit a constant, low-level firing. 3. Gentamicin, a known suppressor of mechanosensory cell activity, blocked the initial rapid burst response to suprathreshold stimuli (n = 11) but had no effect on the adaptive low-level firing. The latency of the delayed activation of C-MHs induced by sustained subthreshold stimuli was not affected by gentamicin. 4. Sphingosine, a protein kinase inhibitor, increased the latency of the delayed activation of C-MHs (n = 7) to sustained subthreshold stimuli; phorbol 12-myristate 13-acetate (TPA), a protein kinase C activator, decreased the latency of the delayed activation of C-MHs (n = 9); and 4 alpha-phorbol, an inactive isomer of TPA, had no effect on the latency of the delayed activation (n = 7). Sphingosine, TPA, and 4 alpha-phorbol had no affect on the initial burst response induced by suprathreshold stimuli. 5. K+ channel blockers, 4-aminopyridine (n = 9) and noxiustoxin (n = 5), decreased the latency of the delayed activation of C-MHs to sustained subthreshold stimuli but had no effect on the initial burst response of C-MHs to suprathreshold stimuli.(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/jn.1991.66.2.363","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T21:38:38Z","timestamp":1514151518000},"page":"363-368","source":"Crossref","is-referenced-by-count":8,"title":["Different mechanical transduction mechanisms for the immediate and delayed responses of rat C-fiber nociceptors"],"prefix":"10.1152","volume":"66","author":[{"given":"D. M.","family":"White","sequence":"first","affiliation":[{"name":"Department of Medicine, University of California, San Francisco94143."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. D.","family":"Levine","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco94143."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["Journal of Neurophysiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jn.1991.66.2.363","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T05:17:57Z","timestamp":1568006277000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jn.1991.66.2.363"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,8,1]]}},"alternative-id":["10.1152\/jn.1991.66.2.363"],"URL":"https:\/\/doi.org\/10.1152\/jn.1991.66.2.363","relation":{},"ISSN":["0022-3077","1522-1598"],"issn-type":[{"value":"0022-3077","type":"print"},{"value":"1522-1598","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,8,1]]}}}