{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T03:39:02Z","timestamp":1773459542727,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1993,10,1]]},"abstract":"<jats:p> 1. Whole-cell and cell-attached patch-clamp recordings were made from enzymatically isolated type I cells from the carotid body of adult rats. Voltage-dependent K+ and Ca2+ channels were observed, but there was no detectable Na+ current. In this respect, rat carotid body cells are unlike those from rabbit, which have Na+ currents and Na(+)-dependent action potentials. 2. The observed Ca2+ channels had the following properties: 1) activation requires voltage steps above -20 mV; 2) little inactivation occurred with holding voltages below -40 mV; 3) one single-channel conductance of 21 pS was found with 90 or 110 mM Ba2+ in the cell-attached pipette and this was the only conductance observed; 4) open probability was increased by the dihydropyridine Ca2+ channel agonist Bay K 8644 and was decreased by the antagonist nifedipine; and 5) omega-conotoxin had little or no effect on the channels. These are properties expected of L-type Ca2+ channels. 3. To investigate whether these voltage-dependent channels would be available for opening on membrane depolarization, we measured the type I cell resting membrane potential noninvasively using unitary openings of the L-type Ca2+ channel with Bay K 8644 in the cell-attached pipette. Resting potentials ranged from -62 to -13 mV, with a mean of -32 mV in 12 cells. 4. Judging from single-channel conductance and pharmacology, the Ca2+ current is mostly, if not solely, carried by L channels. Thus it should be possible to use modulators of L channel activity to determine the role of Ca2+ channels in stimulus-secretion coupling in the rat carotid body. <\/jats:p>","DOI":"10.1152\/jn.1993.70.4.1378","type":"journal-article","created":{"date-parts":[[2017,12,25]],"date-time":"2017-12-25T02:27:25Z","timestamp":1514168845000},"page":"1378-1384","source":"Crossref","is-referenced-by-count":41,"title":["L-type calcium channels in type I cells of the rat carotid body"],"prefix":"10.1152","volume":"70","author":[{"given":"L. A.","family":"Fieber","sequence":"first","affiliation":[{"name":"Washington University School of Medicine, Department of Cell Biologyand Physiology, St. Louis, Missouri 63110."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. W.","family":"McCleskey","sequence":"additional","affiliation":[{"name":"Washington University School of Medicine, Department of Cell Biologyand Physiology, St. Louis, Missouri 63110."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["Journal of Neurophysiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jn.1993.70.4.1378","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T05:16:31Z","timestamp":1568006191000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jn.1993.70.4.1378"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1993,10,1]]}},"alternative-id":["10.1152\/jn.1993.70.4.1378"],"URL":"https:\/\/doi.org\/10.1152\/jn.1993.70.4.1378","relation":{},"ISSN":["0022-3077","1522-1598"],"issn-type":[{"value":"0022-3077","type":"print"},{"value":"1522-1598","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,10,1]]}}}