{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T22:26:56Z","timestamp":1790202416491,"version":"4.1.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1995,6,1]]},"abstract":"<jats:p> 1. Whole cell recordings from layer V neurons of mouse somatosensory cortex were made with the use of a \"blind\" patch-clamp technique. In slices from immature [postnatal days 6 to 11 (P6-P11)] and juvenile (P18-P21) animals, inhibitory postsynaptic currents (IPSCs) were evoked in all cells by extracellular stimulation at the layer V-VI border. Monosynaptic IPSCs, with latency &lt; 2 ms, were isolated pharmacologically by blockade of ionotropic glutamatergic transmission. IPSCs were blocked by bicuculline methiodide and reversed near the predicted equilibrium potential for Cl-. 2. IPSC characteristics were not different for the two age groups. At 1.5-2 times threshold intensity (0.2 Hz), they fluctuated in amplitude with occasional failures. At -70 or -80 mV, mean amplitudes were -202 +\/- 20 (SE) pA and -207 +\/- 32 pA for immature (39 cells) and juvenile (13 cells) cortex, respectively. Half rise times were 0.74 +\/- 0.03 ms (n = 7 cells) in neonates and 0.67 +\/- 0.04 ms (n = 7 cells) in juveniles. Decays were biexponential with tau 1 = 14.8 +\/- 1.3 ms and tau 2 = 59.0 +\/- 7.4 ms (n = 7 cells) in neonates, and tau 1 = 11.9 +\/- 1.1 ms and tau 2 = 55.5 +\/- 4.2 ms (n = 7 cells) in juveniles. 3. Pairs of stimuli elicited paired-pulse facilitation (PPF) when delivered at brief interstimulus intervals (ISI), and paired-pulse depression (PPD) at long ISI. PPF, which was evident in 64% of immature cells and 38% of juvenile cells, was maximal (38 +\/- 4% greater than the conditioning response) at 20-40 ms. PPD, which was evident in 82% of immature cells and 87% of juvenile cells, was maximal (29 +\/- 2% smaller than the conditioning response) by 300 ms. In each age group, some animals showed PPF without PPD.(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/jn.1995.73.6.2591","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T18:23:09Z","timestamp":1514139789000},"page":"2591-2595","source":"Crossref","is-referenced-by-count":25,"title":["Paired-pulse facilitation of IPSCs in slices of immature and mature mouse somatosensory neocortex"],"prefix":"10.1152","volume":"73","author":[{"given":"I. A.","family":"Fleidervish","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Health Sciences, Ben-GurionUniversity of the Negev, Beersheva, Israel."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. J.","family":"Gutnick","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Health Sciences, Ben-GurionUniversity of the Negev, Beersheva, Israel."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["Journal of Neurophysiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jn.1995.73.6.2591","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T01:19:01Z","timestamp":1567991941000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jn.1995.73.6.2591"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1995,6,1]]}},"alternative-id":["10.1152\/jn.1995.73.6.2591"],"URL":"https:\/\/doi.org\/10.1152\/jn.1995.73.6.2591","relation":{},"ISSN":["0022-3077","1522-1598"],"issn-type":[{"value":"0022-3077","type":"print"},{"value":"1522-1598","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,6,1]]}}}