{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T20:01:19Z","timestamp":1762113679033},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1995,9,1]]},"abstract":"<jats:p> 1. The tight-seal whole cell recording technique was used to study the effects of the metabotropic glutamate receptor (mGluR) agonist, trans-1-aminocyclopentane-1,3-dicarboxylic acid (t-ACPD) on spontaneous gamma-aminobutyric acid (GABA)-mediated synaptic currents in neonatal rat CA1 hippocampal neurons in slices obtained from postnatal (P) days P6-P12. 2. Bath application of t-ACPD (3-30 microM), in the presence of kynurenic acid, induced a concentration-dependent increase in frequency but not in amplitude of spontaneous GABAergic currents. The mean frequency ratio (t-ACPD 10 microM over control) was 2.6 +\/- 1 (mean +\/- SD), whereas the mean amplitude ratio was 1.1 +\/- 0.3. 3. The effect of t-ACPD was partially antagonized by the mGluR antagonist (RS)-alpha-methyl-4-carboxyphenylglycine (MCPG, 1 mM). 4. t-ACPD (10-30 microM) did not modify the frequency of miniature GABAergic synaptic currents recorded in tetrodotoxin (the mean frequency ratio of t-ACPD over control was 0.7 +\/- 0.3). 5. Forskolin (30 microM), but not its analogue 1,9 dideoxyforskolin (30 microM), mimicked the effect of t-ACPD. Similar effects were obtained with 3-isobutyl-1-methylxanthine (IBMX, 200 microM). 6. The potentiating effect of t-ACPD on spontaneous GABAergic currents was prevented by Rp-cAMPS (30 microM), a specific antagonist of protein kinase A. This suggests that mGluRs localized at the soma-dendritic level of GABAergic interneurons and positively coupled to cyclic AMP may modulate GABA release during a critical period of postnatal development. <\/jats:p>","DOI":"10.1152\/jn.1995.74.3.1118","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T23:18:29Z","timestamp":1514157509000},"page":"1118-1122","source":"Crossref","is-referenced-by-count":14,"title":["Activation of metabotropic glutamate receptors increase the frequency of spontaneous GABAergic currents through protein kinase A in neonatal rat hippocampal neurons"],"prefix":"10.1152","volume":"74","author":[{"given":"M.","family":"Sciancalepore","sequence":"first","affiliation":[{"name":"Biophysics Laboratory, International School for Advanced Studies(SISSA), Trieste, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Stratta","sequence":"additional","affiliation":[{"name":"Biophysics Laboratory, International School for Advanced Studies(SISSA), Trieste, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. D.","family":"Fisher","sequence":"additional","affiliation":[{"name":"Biophysics Laboratory, International School for Advanced Studies(SISSA), Trieste, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Cherubini","sequence":"additional","affiliation":[{"name":"Biophysics Laboratory, International School for Advanced Studies(SISSA), Trieste, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["Journal of Neurophysiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jn.1995.74.3.1118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T05:20:15Z","timestamp":1568006415000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jn.1995.74.3.1118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,9,1]]}},"alternative-id":["10.1152\/jn.1995.74.3.1118"],"URL":"https:\/\/doi.org\/10.1152\/jn.1995.74.3.1118","relation":{},"ISSN":["0022-3077","1522-1598"],"issn-type":[{"value":"0022-3077","type":"print"},{"value":"1522-1598","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,9,1]]}}}