{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:10:25Z","timestamp":1758924625477},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1996,10,1]]},"abstract":"<jats:p> 1. As previously reported, in hippocampal slices from Sprague-Dawley rats, 13-min applications of 2-deoxy-D-glucose (2-DG) (substituting 10 mM 2-DG for glucose)-which sharply depress field excitatory postsynaptic potentials (EPSPs)-are followed by a sustained potentiation of the initial slopes of EPSPs (2-DG-LTP). 2. Such 2-DG-LTP is not prevented by exposing slices to Ca(2+)-free medium for 25 min before the 13-min 2-DG applications (in Ca(2+)-free medium). Therefore 2-DG-LTP is not dependent on influx of external Ca2+ during the 2-DG applications. 3. When the Ca(2+)-free conditions begin 15 min before, and are maintained for 10 min after, the 13-min 2-DG applications (in Ca(2+)-free medium), 2-DG-LTP is either totally suppressed or much reduced. A delayed Ca2+ influx thus plays a crucial role in the induction of 2-DG-LTP. 4. Much longer Ca(2+)-free pretreatment (for 77 min) largely abolishes 2-DG-LTP. Therefore Ca2+ release from a compartment (presumably intracellular) that is not readily depleted is also important for the induction of 2-DG-LTP. 5. This intracellular Ca2+ store is sensitive to dantrolene sodium (10 microM)-which prevents 2-DG-LTP-but not 10 microM thapsigargin. 2-DG-LTP of isolated N-methyl-D-aspartate-receptor-mediated EPSPs is only partly reduced by dantrolene. 6. Dantrolene (10 microM) also reduces or abolishes posttetanic potentiation, but not paired-pulse facilitation. 7. Depotentiation by 1-Hz stimulation is abolished by 20 microM dantrolene. 8. In contrast to the above, long-term potentiation (LTP) elicited by tetanic stimulation is prevented by 10 microM thapsigargin but not by dantrolene (&lt; or = 50 microM). 9. In conclusion, two mechanisms of intracellular Ca2+ concentration increase appear to be essential for LTP induction by 2-DG. One is Ca2+ influx after the 2-DG application; the other is Ca2+ release from a dantrolene-sensitive internal store. The opposite effects of thapsigargin and dantrolene on 2-DG-LTP and tetanic LTP suggest that distinct internal sources of Ca2+ may be needed for the induction of these two forms of LTP. <\/jats:p>","DOI":"10.1152\/jn.1996.76.4.2343","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T19:23:37Z","timestamp":1514143417000},"page":"2343-2352","source":"Crossref","is-referenced-by-count":24,"title":["Calcium dependence of LTP induced by 2-deoxyglucose in CA1 neurons"],"prefix":"10.1152","volume":"76","author":[{"given":"S.","family":"Tekkok","sequence":"first","affiliation":[{"name":"Anaesthesia Research and Physiology Departments, McGill University,Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Krnjevic","sequence":"additional","affiliation":[{"name":"Anaesthesia Research and Physiology Departments, McGill University,Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["Journal of Neurophysiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jn.1996.76.4.2343","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T01:30:32Z","timestamp":1567992632000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jn.1996.76.4.2343"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1996,10,1]]}},"alternative-id":["10.1152\/jn.1996.76.4.2343"],"URL":"https:\/\/doi.org\/10.1152\/jn.1996.76.4.2343","relation":{},"ISSN":["0022-3077","1522-1598"],"issn-type":[{"value":"0022-3077","type":"print"},{"value":"1522-1598","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,10,1]]}}}