{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T17:26:54Z","timestamp":1777483614861,"version":"3.51.4"},"reference-count":43,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1997,3,1]]},"abstract":"<jats:p> Beck, H., R. Steffens, U. Heinemann, and C.\u2009E. Elger. Properties of voltage-activated Ca<jats:sup>2+<\/jats:sup> currents in acutely isolated human hippocampal granule cells. J. Neurophysiol. 77: 1526\u20131537, 1997. Properties of Ba<jats:sup>2+<\/jats:sup> currents through voltage-dependent Ca<jats:sup>2+<\/jats:sup> channels ( I <jats:sub>Ba<\/jats:sub>) were investigated in 61 dentate granule cells acutely isolated from the resected hippocampus of nine patients with therapy-refractory temporal lobe epilepsy (TLE). Currents with a high threshold of activation (HVA) peaked at 0 mV, and showed some time-dependent inactivation and a voltage of half-maximal steady-state inactivation ( V <jats:sub>1\/2inact<\/jats:sub>) of \u221216.4 mV. Application of saturating doses of \u03c9-conotoxin (\u03c9-CgTx) GVIA or nifedipine distinguished characteristic N-type (38%) and L-type (62% of HVA currents) Ca<jats:sup>2+<\/jats:sup> currents. Combined application of both agents blocked HVA currents by &gt;95%. In a 10-mo-old child but not in adult patients, an \u03c9-agatoxin IVA (\u03c9-AgaTx IVA)-sensitive but \u03c9-CgTx MVIIC-insensitive, noninactivating component of HVA currents (\u223c24%) was present that most probably corresponds to a P-type current. A T-type Ca<jats:sup>2+<\/jats:sup> current could be separated from HVA components on the basis of its steady-state voltage-dependent inactivation( V <jats:sub>1\/2inact<\/jats:sub> = \u221271.0 mV). The T-type Ca<jats:sup>2+<\/jats:sup> current isolated by subtraction peaked at more negative potentials (\u221210 mV), showed a significantly more rapid time-dependent inactivation, and could be selectively blocked by low concentrations of Ni<jats:sup>2+<\/jats:sup>. It was insensitive to nifedipine and \u03c9-CgTx GVIA. We conclude that L-, N-, and T-type currents are present in adult human dentate granule cells and an additional P-type current is present in neurons from a 10-mo-old patient. These data may provide a basis for comparison with animal models of epilepsy and for the elucidation of mechanisms of action of drugs intended for use in human disease. <\/jats:p>","DOI":"10.1152\/jn.1997.77.3.1526","type":"journal-article","created":{"date-parts":[[2017,12,25]],"date-time":"2017-12-25T00:48:16Z","timestamp":1514162896000},"page":"1526-1537","source":"Crossref","is-referenced-by-count":47,"title":["Properties of Voltage-Activated Ca<sup>2+<\/sup> Currents in Acutely Isolated Human Hippocampal Granule Cells"],"prefix":"10.1152","volume":"77","author":[{"given":"H.","family":"Beck","sequence":"first","affiliation":[{"name":"Department of Epileptology, University of Bonn Medical Center, D-53105 Bonn, Germany; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Steffens","sequence":"additional","affiliation":[{"name":"Department of Epileptology, University of Bonn Medical Center, D-53105 Bonn, Germany; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U.","family":"Heinemann","sequence":"additional","affiliation":[{"name":"Department of Neurophysiology, Institute of Physiology, Charit\u00e9 Berlin, D-10000 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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