{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T01:11:01Z","timestamp":1793149861871,"version":"4.5.0"},"reference-count":40,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1997,4,1]]},"abstract":"<jats:p>L\u00fcckermann, Mark, Stefan Trapp, and Klaus Ballanyi. GABA- and glycine-mediated fall of intracellular pH in rat medullary neurons in situ. J. Neurophysiol. 77: 1844\u20131852, 1997. In the region of the ventral respiratory group in brain stem slices from neonatal rats, intracellular pH (pH<jats:sub>i<\/jats:sub>) and membrane currents ( I<jats:sub>m<\/jats:sub>) or potentials were measured in neurons dialyzed with the pH-sensitive dye 2\u2032,7\u2032-bis-carboxyethyl-5(6)-carboxyfluorescein. Currents and increases in membrane conductance ( g<jats:sub>m<\/jats:sub>) during bath application of 0.1 or 1 mM \u03b3-aminobutyric acid (GABA) were accompanied by a delayed mean fall of pH<jats:sub>i<\/jats:sub>by 0.17 and 0.25 pH units, respectively, from a pH<jats:sub>i<\/jats:sub>baseline of 7.33. These effects were reversibly suppressed by 50\u2013100 \u03bcM bicuculline. Similar effects on I<jats:sub>m<\/jats:sub>, g<jats:sub>m<\/jats:sub>, and pH<jats:sub>i<\/jats:sub>were revealed on administration of 0.1 or 1 mM glycine. These responses were abolished by 10\u2013100 \u03bcM strychnine. Dialysis of the cells with 15\u201330 \u03bcM carbonic anhydrase led to an acceleration of the kinetics and a potentiation of the GABA-induced pH<jats:sub>i<\/jats:sub>decrease. GABA- and glycine-evoked pH<jats:sub>i<\/jats:sub>decreases were very similar during recordings with either high- or low-Cl<jats:sup>\u2212<\/jats:sup>patch electrodes, although the reversal potential of the accompanying currents differed by \u223c60 mV. The GABA-induced pH<jats:sub>i<\/jats:sub>decrease, but not the accompanying I<jats:sub>m<\/jats:sub>and g<jats:sub>m<\/jats:sub>responses, was suppressed in CO<jats:sub>2<\/jats:sub>\/HCO<jats:sup>\u2212<\/jats:sup><jats:sub>3<\/jats:sub>-free, N-2-hydroxy-ethylpiperazine- N\u2032-2-ethane sulphonic acid pH-buffered solution. Depolarization from \u221260 to +30 mV resulted in a sustained fall of pH<jats:sub>i<\/jats:sub>by maximally 0.5 pH units. In this situation, the GABA-induced fall of pH<jats:sub>i<\/jats:sub>turned into an intracellular alkalosis of 0.09\u20130.15 pH units. The results confirm and extend previous findings obtained in vivo that GABA- or glycine-induced intracellular acidosis of respiratory neurons is due to efflux of HCO<jats:sup>\u2212<\/jats:sup><jats:sub>3<\/jats:sub>via the receptor-coupled Cl<jats:sup>\u2212<\/jats:sup>channel.<\/jats:p>","DOI":"10.1152\/jn.1997.77.4.1844","type":"journal-article","created":{"date-parts":[[2017,12,25]],"date-time":"2017-12-25T06:02:13Z","timestamp":1514181733000},"page":"1844-1852","source":"Crossref","is-referenced-by-count":33,"title":["GABA- and Glycine-Mediated Fall of Intracellular pH in Rat Medullary Neurons In Situ"],"prefix":"10.1152","volume":"77","author":[{"given":"Mark","family":"L\u00fcckermann","sequence":"first","affiliation":[{"name":"II. Physiologisches Institut, Universit\u00e4t G\u00f6ttingen, D-37073 G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Trapp","sequence":"additional","affiliation":[{"name":"II. 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