{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T01:22:28Z","timestamp":1789694548792,"version":"4.0.1"},"reference-count":25,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1997,8,1]]},"abstract":"<jats:p>Wu, S.\u2009Y. and N.\u2009J. Dun. Potentiation of NMDA currents by pituitary adenylate cyclase activating polypeptide in neonatal rat sympathetic preganglionic neurons. J. Neurophysiol. 78: 1175\u20131179, 1997. Whole cell patch-clamp recordings were made from sympathetic preganglionic neurons (SPNs) in the intermediolateral cell column of thoracolumbar spinal cord slices of 12- to 16-day-old rats, and the effects of pituitary adenylate cyclase activating polypeptide (PACAP)-38 on N-methyl-d-aspartate (NMDA)- and kainate (KA)-induced inward currents were examined. PACAP, in concentrations (10\u201330 nM) that caused no significant change of holding currents, reversibly increased NMDA-induced currents but not KA-induced currents. At higher concentrations (&gt;30 nM), the peptide produced a sustained inward current. The potentiating effect of PACAP was nullified by prior incubation of the slices with the adenylate cyclase inhibitor MDL-12,330A (25 \u03bcM). Further, superfusing the slices with the membrane-permeable cyclic AMP analogue N<jats:sup>6<\/jats:sup>,2\u2032-0-dibutyryladenosine 3\u2032:5\u2032-cyclic monophosphate (100\u2013300 \u03bcM) in the presence of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (700 \u03bcM) increased the NMDA currents. This result suggests that PACAP selectively increases NMDA-receptor-mediated responses in the rat SPNs, probably via a cyclic-AMP-dependent mechanism, providing evidence that the peptide may be involved in synaptic plasticity.<\/jats:p>","DOI":"10.1152\/jn.1997.78.2.1175","type":"journal-article","created":{"date-parts":[[2017,12,25]],"date-time":"2017-12-25T07:05:12Z","timestamp":1514185512000},"page":"1175-1179","source":"Crossref","is-referenced-by-count":27,"title":["Potentiation of NMDA Currents by Pituitary Adenylate Cyclase Activating Polypeptide in Neonatal Rat Sympathetic Preganglionic Neurons"],"prefix":"10.1152","volume":"78","author":[{"given":"S. Y.","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Anatomy and Neurobiology, Medical College of Ohio, Toledo, Ohio 43614"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. J.","family":"Dun","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neurobiology, Medical College of Ohio, Toledo, Ohio 43614"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(91)90921-C"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(92)90621-Z"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(93)90275-P"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90270-A"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1038\/356521a0"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(96)12882-2"},{"key":"B7","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0031-6997(25)00025-0","volume":"40","author":"Collingridge G. L.","year":"1989","journal-title":"Pharmacol. Rev."},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(96)00139-4"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(96)00057-7"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1126\/science.7754370"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1126\/science.1716001"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13733.x"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(93)90295-V"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1126\/science.7754367"},{"key":"B15","first-page":"209","volume":"99","author":"Lippe C.","year":"1991","journal-title":"Comp. Biochem. Physiol."},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(96)00359-7"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80059-8"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1993.tb03242.x"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1838(90)90151-8"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1210\/endo-128-6-3055"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/0959-4388(91)90018-3"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1038\/365170a0"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1126\/science.1653455"},{"key":"B24","first-page":"1998","volume":"22","author":"Wu S. Y.","year":"1996","journal-title":"Soc. Neurosci. Abstr."},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021608"}],"container-title":["Journal of Neurophysiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jn.1997.78.2.1175","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T05:44:42Z","timestamp":1751175882000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jn.1997.78.2.1175"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,8,1]]},"references-count":25,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,8,1]]}},"alternative-id":["10.1152\/jn.1997.78.2.1175"],"URL":"https:\/\/doi.org\/10.1152\/jn.1997.78.2.1175","relation":{},"ISSN":["0022-3077","1522-1598"],"issn-type":[{"value":"0022-3077","type":"print"},{"value":"1522-1598","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,8,1]]}}}