{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:38:07Z","timestamp":1790642287135,"version":"4.1.0"},"reference-count":31,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1997,11,1]]},"abstract":"<jats:p>Shirahata, Machiko, Robert S. Fitzgerald, and James S.\u2009K. Sham. Acetylcholine increases intracellular calcium of arterial chemoreceptor cells of adult cats. J. Neurophysiol. 78: 2388\u20132395, 1997. Several neurotransmitters have been reported to play important roles in the chemoreception of the carotid body. Among them acetylcholine (ACh) appears to be involved in excitatory processes in the cat carotid body. As one of the steps to elucidate possible roles of ACh in carotid body chemoreception in the cat, we examined the effect of ACh on intracellular calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) of cultured carotid body cells. The carotid body from adult cats was dissociated and cultured for up to 2 wk. [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>was measured from clusters of cells with a microfluorometric technique using Indo-1 AM. Experiments were performed at 37\u00b0C, and cells were continuously superfused with modified Krebs solutions equilibrated with 5% CO<jats:sub>2<\/jats:sub>-16% O<jats:sub>2<\/jats:sub>-79% N<jats:sub>2<\/jats:sub>. ACh (100 \u03bcM) caused a marked increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in \u223c70% of clusters, and the responses to 1\u2013300 \u03bcM of ACh were concentration dependent. The magnitude and kinetics of the ACh response were mimicked by the application of nicotine, whereas muscarinic agonists, pilocarpine, and muscarine failed to evoke a similar response. ACh-induced increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>was dependent on extracellular Ca<jats:sup>2+<\/jats:sup>: it was greatly reduced or completely abolished by a transient removal of extracellular Ca<jats:sup>2+<\/jats:sup>. The response was consistently but only partially reduced by caffeine (5 mM) or nifedipine (10 \u03bcM). The effect of mecamylamine (100 \u03bcM) was inhibitory but small. Moreover, the increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in response to ACh was also observed in some clusters that did not respond to high K (100 mM) Krebs. These results suggest that ACh increases [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>of cultured carotid body cells by activating neuronal nicotinic ACh receptors, leading to Ca<jats:sup>2+<\/jats:sup>influx via nicotinic channels. In addition, other pathways such as Ca<jats:sup>2+<\/jats:sup>influx through L-type calcium channels, perhaps secondary to membrane depolarization, and Ca<jats:sup>2+<\/jats:sup>release from intracellular stores may participate in increasing [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in response to ACh. Muscarinic receptors appear to play only a small role, if\u2009any.<\/jats:p>","DOI":"10.1152\/jn.1997.78.5.2388","type":"journal-article","created":{"date-parts":[[2017,12,25]],"date-time":"2017-12-25T06:14:15Z","timestamp":1514182455000},"page":"2388-2395","source":"Crossref","is-referenced-by-count":23,"title":["Acetylcholine\u2009Increases\u2009Intracellular\u2009Calcium\u2009of\u2009Arterial\u2009Chemoreceptor Cells of Adult Cats"],"prefix":"10.1152","volume":"78","author":[{"given":"Machiko","family":"Shirahata","sequence":"first","affiliation":[{"name":"Department of Environmental Health Sciences,"},{"name":"Department of Anesthesiology and Critical Care Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert S.","family":"Fitzgerald","sequence":"additional","affiliation":[{"name":"Department of Environmental Health Sciences,"},{"name":"Department of Physiology, and"},{"name":"Department of Medicine, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James S. 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