{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T10:56:58Z","timestamp":1681815418301},"reference-count":42,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1998,2,1]]},"abstract":"<jats:p> Kloub, Mamoun A., Gerard L. Heck, and John A. DeSimone. Self-inhibition in Ca<jats:sup>2+<\/jats:sup>-evoked taste receptors: a novel tool for functional dissection of salt taste transduction mechanisms. J. Neurophysiol. 79: 911\u2013921, 1998. Rat chorda tympani (CT) responses to CaCl<jats:sub>2<\/jats:sub> were obtained during simultaneous current and voltage clamping of the lingual receptive field. Unlike most other salts, CaCl<jats:sub>2<\/jats:sub> induced negatively directed transepithelial potentials and gave CT responses that were auto-inhibitory beyond a critical concentration. CT responses increased in a dose-dependent manner to \u223c0.3 M, whereafter they decreased with increasing concentration. At concentrations where Ca<jats:sup>2+<\/jats:sup> was self-inhibitory, it also inhibited responses to NaCl, KCl, and NH<jats:sub>4<\/jats:sub>Cl present in mixtures with CaCl<jats:sub>2<\/jats:sub>. Ca<jats:sup>2+<\/jats:sup> completely blocked the amiloride-insensitive component of the NaCl CT response, the entire KCl-evoked CT response, and the high-concentration-domain CT responses of NH<jats:sub>4<\/jats:sub>Cl (\u22650.3 M). The overlapping Ca<jats:sup>2+<\/jats:sup>-sensitivity between the responses of the three Cl<jats:sup>\u2212<\/jats:sup> salts (Na<jats:sup>+<\/jats:sup>, K<jats:sup>+<\/jats:sup>, and NH<jats:sup>+<\/jats:sup> <jats:sub>4<\/jats:sub>) suggests a common, Ca<jats:sup>2+<\/jats:sup>-sensitive, transduction pathway. Extracellular Ca<jats:sup>2+<\/jats:sup> has been shown to modulate the paracellular pathways in different epithelial cell lines by decreasing the water permeability and cation conductance of tight junctions. Ca<jats:sup>2+<\/jats:sup>-induced modulation of tight junctions is associated with Ca<jats:sup>2+<\/jats:sup> binding to fixed negative sites. This results in a conversion of ion selectivity from cationic to anionic, which we also observed in our system through simultaneous monitoring of the transepithelial potential during CT recording. The data indicate the paracellular pathway as the stimulatory and modulatory site of CaCl<jats:sub>2<\/jats:sub> taste responses. In addition, they indicate that important transduction sites for NaCl, KCl, and NH<jats:sub>4<\/jats:sub>Cl taste reception are accessible only through the paracellular pathways. More generally, they show that modulation of paracellular transport by Ca<jats:sup>2+<\/jats:sup> in an intact epithelium has functional consequences at a systemic level. <\/jats:p>","DOI":"10.1152\/jn.1998.79.2.911","type":"journal-article","created":{"date-parts":[[2017,12,25]],"date-time":"2017-12-25T07:07:11Z","timestamp":1514185631000},"page":"911-921","source":"Crossref","is-referenced-by-count":8,"title":["Self-Inhibition in Ca<sup>2+<\/sup>-Evoked Taste Responses: A Novel Tool for Functional Dissection of Salt Taste Transduction Mechanisms"],"prefix":"10.1152","volume":"79","author":[{"given":"Mamoun A.","family":"Kloub","sequence":"first","affiliation":[{"name":"Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298-0551"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerard L.","family":"Heck","sequence":"additional","affiliation":[{"name":"Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298-0551"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John A.","family":"Desimone","sequence":"additional","affiliation":[{"name":"Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298-0551"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01871001"},{"issue":"3","key":"B2","first-page":"F234","volume":"235","author":"Berry C. 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