{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T07:17:43Z","timestamp":1791011863365,"version":"4.1.0"},"reference-count":28,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1999,1,1]]},"abstract":"<jats:p> Lape, Remigijus and Andrea Nistri. Voltage-activated K<jats:sup>+<\/jats:sup> currents of hypoglossal motoneurons in a brain stem slice preparation from the neonatal rat. J. Neurophysiol. 81: 140\u2013148, 1999. Whole cell, patch-clamp recordings were performed on motoneurons of the hypoglossus nucleus in a brain stem slice preparation from the neonatal rat brain. The aim was to investigate transient outward currents activated by membrane depolarization under voltage clamp conditions. In a Ca<jats:sup>2+<\/jats:sup>-free medium containing tetrodotoxin and Cs<jats:sup>+<\/jats:sup>, depolarizing voltage commands from a holding potential of \u221250 mV induced slow outward currents ( I <jats:sub>slow<\/jats:sub>) with 34 \u00b1 6 ms (SE) onset time constant at 0 mV and minimal decline during a 1 s pulse depolarization. When the depolarizing command was preceded by a prepulse to \u2212110 mV, the outward current became biphasic as it comprised a faster component ( I <jats:sub>fast<\/jats:sub>), which could be investigated in isolation by subtracting the two sets of records. I <jats:sub>fast<\/jats:sub> showed rapid kinetics (9 \u00b1 4 ms 10\u201390% rise time and 70 \u00b1 20 ms decay time constant at 0 mV) and strong voltage-dependent inactivation (half inactivation was at \u221292.9 \u00b1 0.2 mV) from which it readily recovered with a biexponential timecourse (4.4 \u00b1 0.6 and 17 \u00b1 2 ms time constants at \u2212110 mV membrane potential). I <jats:sub>slow<\/jats:sub> was selectively blocked by TEA (10\u201330 mM) while I <jats:sub>fast<\/jats:sub> was preferentially depressed by 2\u20133 mM 4-aminopyridine. Analysis of tail current reversal indicated that both I <jats:sub>slow<\/jats:sub> and I <jats:sub>fast<\/jats:sub> were predominantly due to K<jats:sup>+<\/jats:sup> with minor permeability to Na<jats:sup>+<\/jats:sup> (92\/1 and 50\/1, respectively). These results suggest that membrane depolarization activated distinct K<jats:sup>+<\/jats:sup> conductances that, in view of their largely dissimilar kinetics, are likely to play a differential role in regulating the firing behavior of hypoglossal motoneurons. <\/jats:p>","DOI":"10.1152\/jn.1999.81.1.140","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T23:58:37Z","timestamp":1514159917000},"page":"140-148","source":"Crossref","is-referenced-by-count":27,"title":["Voltage-Activated K<sup>+<\/sup> Currents of Hypoglossal Motoneurons in a Brain Stem Slice Preparation From the Neonatal Rat"],"prefix":"10.1152","volume":"81","author":[{"given":"Remigijus","family":"Lape","sequence":"first","affiliation":[{"name":"Biophysics Sector and Istituto Nazionale Fisica della Materia Unit, International School for Advanced Studies, 34013 Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Nistri","sequence":"additional","affiliation":[{"name":"Biophysics Sector and Istituto Nazionale Fisica della Materia Unit, International School for Advanced Studies, 34013 Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1980.sp013323"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1994.71.1.119"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1971.sp009365"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1971.sp009366"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198712000-00014"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1990.69.4.1509"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1007\/BF00656997"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1952.sp004764"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(89)90156-5"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(89)90468-X"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.73.2.911"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1993.70.1.246"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(85)90082-7"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(88)90033-4"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.73.5.1799"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1997.78.5.2246"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1998.79.2.1081"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp018009"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-09-05652.1994"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-03-02218.1995"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1993.69.6.2137"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1993.69.6.2150"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(94)90105-8"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020850"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(90)90075-F"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-03-00949.1994"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1991.71.2.488"},{"key":"B29","first-page":"73","volume":"1","author":"Willinger M.","year":"1989","journal-title":"J. NIH Res."}],"container-title":["Journal of Neurophysiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jn.1999.81.1.140","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:24:53Z","timestamp":1567988693000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jn.1999.81.1.140"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,1,1]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1999,1,1]]}},"alternative-id":["10.1152\/jn.1999.81.1.140"],"URL":"https:\/\/doi.org\/10.1152\/jn.1999.81.1.140","relation":{},"ISSN":["0022-3077","1522-1598"],"issn-type":[{"value":"0022-3077","type":"print"},{"value":"1522-1598","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,1,1]]}}}