{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T04:47:16Z","timestamp":1778734036823,"version":"3.51.4"},"reference-count":46,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1999,4,1]]},"abstract":"<jats:p>Modulation of N-type Ca<jats:sup>2+<\/jats:sup>channels by intracellular pH in chick sensory neurons. Both physiological and pathological neuronal events, many of which elevate intracellular [Ca<jats:sup>2+<\/jats:sup>], can produce changes in intracellular pH of between 0.15 and 0.5 U, between pH 7.4 and 6.8. N-type Ca<jats:sup>2+<\/jats:sup>channels, which are intimately involved in exocytosis and other excitable cell processes, are sensitive to intracellular pH changes. However, the pH range over which N-type Ca<jats:sup>2+<\/jats:sup>channels are sensitive, and the sensitivity of N-type Ca<jats:sup>2+<\/jats:sup>channels to small changes in intracellular pH, are unknown. We studied the influence of intracellular pH changes on N-type calcium channel currents in dorsal root ganglion neurons, acutely isolated from 14-day-old chick embryos. Intracellular pH was monitored in patch-clamp recordings with the fluorescent dye, BCECF, and manipulated in both the acidic and basic direction by extracellular application of NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup>in the presence and absence of intracellular NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup>. Changes in intracellular pH between 6.6 and 7.5 produced a graded change in Ca<jats:sup>2+<\/jats:sup>current magnitude with no apparent shift in activation potential. Intracellular acidification from pH 7.3 to 7.0 reversibly inhibited Ca<jats:sup>2+<\/jats:sup>currents by 40%. Acidification from pH 7.3 to pH 6.6 reversibly inhibited Ca<jats:sup>2+<\/jats:sup>currents by 65%. Alkalinization from pH 7.3 to 7.5 potentiated Ca<jats:sup>2+<\/jats:sup>currents by approximately 40%. Channels were sensitive to pH<jats:sub>i<\/jats:sub>changes with high intracellular concentrations of the Ca<jats:sup>2+<\/jats:sup>chelator, bis-( o-aminophenoxy)- N,N,N\u2032,N\u2032-tetraacetic acid, which indicates that the effects of pH<jats:sub>i<\/jats:sub>did not involve a Ca<jats:sup>2+<\/jats:sup>-dependent mechanism. These data indicate that N-type Ca<jats:sup>2+<\/jats:sup>channel currents are extremely sensitive to small changes in pH<jats:sub>i<\/jats:sub>in the range produced by both physiological and pathological events. Furthermore, these data suggest that modulation of N-type Ca<jats:sup>2+<\/jats:sup>channels by pH<jats:sub>i<\/jats:sub>may play an important role in physiological processes that produce small changes in pH<jats:sub>i<\/jats:sub>and a protective role in pathological mechanisms that produce larger changes in pH<jats:sub>i<\/jats:sub>.<\/jats:p>","DOI":"10.1152\/jn.1999.81.4.1839","type":"journal-article","created":{"date-parts":[[2017,12,25]],"date-time":"2017-12-25T05:15:19Z","timestamp":1514178919000},"page":"1839-1847","source":"Crossref","is-referenced-by-count":45,"title":["Modulation of N-Type Ca<sup>2+<\/sup>Channels by Intracellular pH in Chick Sensory Neurons"],"prefix":"10.1152","volume":"81","author":[{"given":"Laszlo","family":"Kiss","sequence":"first","affiliation":[{"name":"Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut 06269"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen J.","family":"Korn","sequence":"additional","affiliation":[{"name":"Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut 06269"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.75.4.403"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1994.72.4.1686"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(97)00189-2"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.265.1.C129"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.67.1.91"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1988.255.6.C844"},{"key":"B7","first-page":"A262","volume":"68","author":"Callahan M. 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