{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:41:10Z","timestamp":1773661270483,"version":"3.50.1"},"reference-count":34,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1999,7,1]]},"abstract":"<jats:p> Short-term depression of monosynaptic GABAergic inhibitory postsynaptic currents (IPSCs) evoked between pairs of cultured rat hippocampal neurons was investigated using dual whole cell patch-clamp recordings. Paired stimuli applied to the GABAergic neuron resulted in paired-pulse depression (PPD) of the second IPSC (IPSC<jats:sub>2<\/jats:sub>) at interpulse intervals from 25 to 2,000 ms. CGP 55845A, but not CGP 35348, reduced PPD marginally. Brief paired-pulse applications of exogenous GABA indicated that postsynaptic factors made only minimal contribution to PPD of IPSCs. IPSC<jats:sub>1<\/jats:sub> and PPD was reduced on lowering [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>o<\/jats:sub> and enhanced on increasing [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>o<\/jats:sub>. The potassium-channel blocker 4-aminopyridine (4-AP), which increases presynaptic Ca<jats:sup>2+<\/jats:sup> influx, enhanced IPSC<jats:sub>1<\/jats:sub> and PPD. Chelation of residual Ca<jats:sup>2+<\/jats:sup> in the GABAergic boutons with EGTA-AM enhanced PPD. Stimulation of the presynaptic neuron at frequencies ( f) ranging from 2.5 to 80 Hz resulted in tetanic depression of IPSCs, which declined rapidly and reached a plateau depending on f and [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>o<\/jats:sub>. CGP 55845A decreased tetanic depression in the first part of the train, but this could be overcome with continued stimulation. We show that GABAergic IPSCs are robustly depressed by paired-pulse stimulation in cultured hippocampal neurons. The depression of IPSCs is mainly independent of presynaptic GABA<jats:sub>B<\/jats:sub> receptors and could be caused by depletion of releasable vesicles. Depleted synapses recover with a slow time course, depending on factors that regulate [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in the GABAergic boutons. <\/jats:p>","DOI":"10.1152\/jn.1999.82.1.42","type":"journal-article","created":{"date-parts":[[2017,12,25]],"date-time":"2017-12-25T00:37:23Z","timestamp":1514162243000},"page":"42-49","source":"Crossref","is-referenced-by-count":52,"title":["Activity-Dependent Depression of GABAergic IPSCs in Cultured Hippocampal Neurons"],"prefix":"10.1152","volume":"82","author":[{"given":"Kimmo","family":"Jensen","sequence":"first","affiliation":[{"name":"Department of Physiology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D. 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