{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T23:21:20Z","timestamp":1777418480081,"version":"3.51.4"},"reference-count":48,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1999,12,1]]},"abstract":" Pyramidal cells typically respond to ischemia with initial transient hyperpolarization, which may represent a neuroprotective response. To identify the conductance underlying this hyperpolarization in CA3 pyramidal neurons of rat hippocampal organotypic slice cultures, recordings were obtained using the single-electrode voltage-clamp technique. Brief chemical ischemia (2 mM 2-deoxyglucose and 3 mM NaN3<\/jats:sub>, for 4 min) induced a response mediated by an increase in K+<\/jats:sup> conductance. This current was blocked by intracellular application of the Ca2+<\/jats:sup> chelator, bis-( o-aminophenoxy)- N,N,N\u2032,N\u2032-tetraacetic acid (BAPTA), reduced with low external [Ca2+<\/jats:sup>], and inhibited by a selective L-type Ca2+<\/jats:sup> channel inhibitor, isradipine, consistent with the activation of a Ca2+<\/jats:sup>-dependent K+<\/jats:sup> conductance. Experiments with charybdotoxin (10 nM) and tetraethylammonium (TEA; 1 mM), or with the protein kinase C activator, phorbol 12,13-diacetate (PDAc; 3 \u03bcM), ruled out an involvement of a large conductance\u2013type or an apamin-insensitive small conductance, respectively. In the presence of apamin (1 \u03bcM), however, the outward current was significantly reduced. These results demonstrate that in rat hippocampal CA3 pyramidal neurons an apamin-sensitive Ca2+<\/jats:sup>-dependent K+<\/jats:sup> conductance is activated in response to brief ischemia generating a pronounced outward current. <\/jats:p>","DOI":"10.1152\/jn.1999.82.6.2876","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T12:41:59Z","timestamp":1513860119000},"page":"2876-2882","source":"Crossref","is-referenced-by-count":14,"title":["Apamin-Sensitive Conductance Mediates the K+<\/sup> Current Response During Chemical Ischemia in CA3 Pyramidal Cells"],"prefix":"10.1152","volume":"82","author":[{"given":"Mitsuo","family":"Tanabe","sequence":"first","affiliation":[{"name":"Brain Research Institute, University of Zurich, CH-8057 Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Mori","sequence":"additional","affiliation":[{"name":"Brain Research Institute, University of Zurich, CH-8057 Zurich, 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