{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T09:13:49Z","timestamp":1722935629443},"reference-count":29,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2000,1,1]]},"abstract":"<jats:p> In hippocampal slices, temporary (10\u201320 min) replacement of glucose with 10 mM 2-deoxyglucose is followed by marked and very sustained potentiation of EPSPs (2-DG LTP). To investigate its mechanism, we examined 2-DG's effect in CA1 neurons recorded with sharp 3 M KCl electrodes containing a strong chelator, 50 or 100 mM ethylene glycol-bis(\u03b2-aminoethyl ether)- N, N, N\u2032, N\u2032-tetraacetic acid (EGTA). In most cases, field EPSPs were simultaneously recorded and conventional LTP was also elicited in some cells by tetanic stimulation of stratum radiatum. 2-DG potentiated intracellular EPSP slopes by 48 \u00b1 5.1% (SE) in nine cells recorded with plain KCl electrodes and by 52 \u00b1 6.2% in seven cells recorded with EGTA-containing electrodes. In four of the latter cells, tetanic stimulation (twice 100 Hz for 1 s) failed to evoke LTP (2 \u00b1 1.1%), although field EPSPs were clearly potentiated (by 28 \u00b1 6.9%). Thus unlike tetanic LTP, 2-DG LTP is not readily prevented by postsynaptic intraneuronal injection of EGTA. These findings agree with other evidence that the rise in postsynaptic (somatic) [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> caused by 2-DG is not the principal trigger for the subsequent 2-DG LTP and that it may be a purely presynaptic phenomenon. <\/jats:p>","DOI":"10.1152\/jn.2000.83.1.177","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T12:32:49Z","timestamp":1513859569000},"page":"177-180","source":"Crossref","is-referenced-by-count":7,"title":["2-Deoxyglucose\u2013Induced Long-Term Potentiation in CA1 Is Not Prevented by Intraneuronal Chelator"],"prefix":"10.1152","volume":"83","author":[{"given":"Yong-Tao","family":"Zhao","sequence":"first","affiliation":[{"name":"Anaesthesia Research Department, McGill University, Montreal, Quebec H3G 1Y6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kre\u0161imir","family":"Krnjevi\u0107","sequence":"additional","affiliation":[{"name":"Anaesthesia Research Department, McGill University, Montreal, Quebec H3G 1Y6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1006\/nlme.1998.3842"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1321498"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/361031a0"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1996.75.2.770"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.14.8310"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440110214"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1993.70.1.1"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/0168-0102(91)90070-F"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1992.67.2.401"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90075-O"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/0959-4388(95)80045-X"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1038\/305719a0"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1038\/377115a0"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80336-0"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.73.2.810"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019900"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(93)90139-3"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1126\/science.8105537"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.450040105"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.74.6.2763"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1996.76.4.2343"},{"key":"B22","first-page":"86P","volume":"501","author":"Tekk\u00f6k S.","year":"1997","journal-title":"J. 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