{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:06:33Z","timestamp":1791893193046,"version":"4.3.4"},"reference-count":45,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2000,4,1]]},"abstract":"<jats:p> We investigated the neuronal plasticity in the spinal dorsal horn and its relationship with spinal inhibitory networks using an optical-imaging method that detects neuronal excitation. High-intensity single-pulse stimulation of the dorsal root activating both A and C fibers evoked an optical response in the lamina II (the substantia gelatinosa) of the dorsal horn in transverse slices of 12- to 25-day-old rat spinal cords stained with a voltage-sensitive dye, RH-482. The optical response, reflecting the net neuronal excitation along the slice-depth, was depressed by 28% for more than 1 h after a high-frequency conditioning stimulation of A fibers in the dorsal root (3 tetani of 100 Hz for 1 s with an interval of 10 s). The depression was not induced in a perfusion solution containing an NMDA antagonist,dl-2-amino-5-phosphonovaleric acid (AP5; 30 \u03bcM). In a solution containing the inhibitory amino acid antagonists bicuculline (1 \u03bcM) and strychnine (3 \u03bcM), and also in a low Cl<jats:sup>\u2212<\/jats:sup>solution, the excitation evoked by the single-pulse stimulation was enhanced after the high-frequency stimulation by 31 and 18%, respectively. The enhanced response after conditioning was depotentiated by a low-frequency stimulation of A fibers (0.2\u20131 Hz for 10 min). Furthermore, once the low-frequency stimulation was applied, the high-frequency conditioning could not potentiate the excitation. Inhibitory transmissions thus regulate the mode of synaptic plasticity in the lamina II most likely at afferent terminals. The high-frequency conditioning elicits a long-term depression (LTD) of synaptic efficacy under a greater activity of inhibitory amino acids, but it results in a long-term potentiation (LTP) when inhibition is reduced. The low-frequency preconditioning inhibits the potentiation induction and maintenance by the high-frequency conditioning. These mechanisms might underlie robust changes of nociception, such as hypersensitivity after injury or inflammation and pain relief after electrical or cutaneous stimulation. <\/jats:p>","DOI":"10.1152\/jn.2000.83.4.2412","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T12:59:26Z","timestamp":1513861166000},"page":"2412-2420","source":"Crossref","is-referenced-by-count":48,"title":["Robust Changes of Afferent-Induced Excitation in the Rat Spinal Dorsal Horn After Conditioning High-Frequency Stimulation"],"prefix":"10.1152","volume":"83","author":[{"given":"Hiroshi","family":"Ikeda","sequence":"first","affiliation":[{"name":"Department of Human and Artificial Intelligence Systems, Fukui University, Fukui 910, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Asai","sequence":"additional","affiliation":[{"name":"Department of Human and Artificial Intelligence Systems, Fukui University, Fukui 910, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuyuki","family":"Murase","sequence":"additional","affiliation":[{"name":"Department of Human and Artificial Intelligence Systems, Fukui University, Fukui 910, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/347069a0"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(76)90042-7"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-3623(96)00359-X"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)91600-6"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1972.sp009767"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1999.82.4.1957"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)00928-7"},{"key":"B13","first-page":"393","volume":"28","author":"Ikeda H.","year":"1998","journal-title":"Soc. 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