{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:00:41Z","timestamp":1791352841491,"version":"4.3.1"},"reference-count":48,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2000,12,1]]},"abstract":"<jats:p>The biophysical and pharmacological properties of voltage-gated calcium channel currents in the human teratocarcinoma cell line NT2-N were studied using the whole cell patch-clamp technique. When held at \u221280 mV, barium currents ( I<jats:sub>Ba<\/jats:sub>s) were evoked by voltage commands to above \u221235 mV that peaked at +5 mV. When holding potentials were reduced to \u221220 mV or 5 mM barium was substituted for 5 mM calcium, there was a reduction in peak currents and a right shift in the current-voltage curve. A steady-state inactivation curve for I<jats:sub>Ba<\/jats:sub>was fit with a Boltzmann curve ( V<jats:sub>1\/2<\/jats:sub>= \u221243.3 mV; slope = \u221217.7 mV). Maximal current amplitude increased from 1-wk (232 pA) to 9-wk (1025 pA) postdifferentiation. Whole cell I<jats:sub>Ba<\/jats:sub>s were partially blocked by specific channel blockers to a similar extent in 1- to 3-wk and 7- to 9-wk postdifferentiation NT2-N cells: 10 \u03bcM nifedipine (19 vs. 25%), 10 \u03bcM conotoxin GVIA (27 vs. 25%), 10 \u03bcM conotoxin MVIIC (15 vs. 16%), and 1.75 \u03bcM SNX-482 (31 vs. 33%). Currents were completely blocked by 300 \u03bcM cadmium. In the presence of nifedipine, GVIA, and MVIIC, \u223c35% of current remained, which was reduced further by SNX-482 (7\u201314% of current remained), consistent with functional expression of L-, N-, and P\/Q-calcium channel types and one or more R-type channel. The presence of multiple calcium currents in this human neuronal-type cell line provides a potentially useful model for study of the regulation, expression and cellular function of human derived calcium channel currents; in particular the R-type current(s).<\/jats:p>","DOI":"10.1152\/jn.2000.84.6.2933","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T13:42:11Z","timestamp":1513863731000},"page":"2933-2944","source":"Crossref","is-referenced-by-count":44,"title":["Functional Expression of L-, N-, P\/Q-, and R-Type Calcium Channels in the Human NT2-N Cell Line"],"prefix":"10.1152","volume":"84","author":[{"given":"Torben R.","family":"Neelands","sequence":"first","affiliation":[{"name":"Neuroscience Program,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony P. 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