{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:22:17Z","timestamp":1791296537606,"version":"4.1.0"},"reference-count":38,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2001,7,1]]},"abstract":"<jats:p>The physiological effects of 5HT receptor coupling to TTX-resistant Na<jats:sup>+<\/jats:sup>current, and the signaling pathway involved, was studied in a nociceptor-like subpopulation of rat dorsal root ganglion (DRG) cells (type 2), which can be identified by expression of a low-threshold, slowly inactivating A-current. The 5HT-mediated increase in TTX-resistant Na<jats:sup>+<\/jats:sup>current in type 2 DRG cells was mimicked and occluded by 10 \u03bcM forskolin. Superfusion of type 2 DRG cells on the outside with 1 mM 8-bromo-cAMP or chlorophenylthio-cAMP (CPT-cAMP) increased the Na<jats:sup>+<\/jats:sup>current, but less than 5HT itself. However, perfusion of the cells inside with 2 mM CPT-cAMP strongly increased the amplitude of control Na<jats:sup>+<\/jats:sup>currents and completely occluded the effect of 5HT. Thus it appears that the signaling pathway includes cAMP. The phosphodiesterase inhibitor 3-isobutyl-l-methylxanthine (200 \u03bcM) also mimicked the effect of 5HT on Na<jats:sup>+<\/jats:sup>current, suggesting tonic adenylyl cyclase activity. 5HT reduced the amount of current required to evoke action potentials in type 2 DRG cells, suggesting that 5HT may lower the threshold for activation of nociceptor peripheral receptors. The above data suggest that serotonergic modulation of TTX-resistant Na<jats:sup>+<\/jats:sup>channels through a cAMP-dependent signaling pathway in nociceptors may participate in the generation of hyperalgesia.<\/jats:p>","DOI":"10.1152\/jn.2001.86.1.241","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T10:54:56Z","timestamp":1513853696000},"page":"241-248","source":"Crossref","is-referenced-by-count":78,"title":["5HT Increases Excitability of Nociceptor-Like Rat Dorsal Root Ganglion Neurons Via cAMP-Coupled TTX-Resistant Na<sup>+<\/sup>Channels"],"prefix":"10.1152","volume":"86","author":[{"given":"Luz M.","family":"Cardenas","sequence":"first","affiliation":[{"name":"Department of Anatomy and Neurobiology, Health Science Center, University of Tennessee, Memphis, Tennessee 38163"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla G.","family":"Cardenas","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neurobiology, Health Science Center, University of Tennessee, Memphis, Tennessee 38163"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reese S.","family":"Scroggs","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neurobiology, Health Science Center, University of Tennessee, Memphis, Tennessee 38163"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00592598"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(92)90051-7"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(94)90059-0"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-19-07181.1997"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.74.5.1870"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0507p.x"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1996.76.4.2192"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711040103"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.857ba.x"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(97)00066-3"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.05-12-03278.1985"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1957.sp005836"},{"key":"B13","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1016\/S0026-895X(25)10130-2","volume":"34","author":"Dumuis A","year":"1988","journal-title":"Mol Pharmacol"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021604"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)01510-2"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1992.tb09087.x"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0433v.x"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(92)90272-6"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-24-10345.1998"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.3.1108"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1985.sp015573"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1985.sp015574"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(92)90115-R"},{"key":"B25","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0031-6997(25)06664-5","volume":"43","author":"Holzer P","year":"1991","journal-title":"Pharmacol Rev"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(90)90142-R"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(90)90141-Q"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1007\/BF00167212"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901860203"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901860202"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(78)90308-4"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1084\/jem.103.4.399"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.771bg.x"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp018944"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(65)90147-5"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(92)90507-X"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(92)90508-Y"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1996.76.3.1924"},{"key":"B40","first-page":"118","volume":"46","author":"Vinegar R","year":"1987","journal-title":"Federation Proc"}],"container-title":["Journal of Neurophysiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jn.2001.86.1.241","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T03:31:03Z","timestamp":1751167863000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jn.2001.86.1.241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,7,1]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,7,1]]}},"alternative-id":["10.1152\/jn.2001.86.1.241"],"URL":"https:\/\/doi.org\/10.1152\/jn.2001.86.1.241","relation":{},"ISSN":["0022-3077","1522-1598"],"issn-type":[{"value":"0022-3077","type":"print"},{"value":"1522-1598","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,7,1]]}}}