{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T14:49:51Z","timestamp":1758638991482,"version":"3.41.0"},"reference-count":49,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2001,11,1]]},"abstract":"<jats:p>The brain is particularly sensitive to alcohol during the period of its rapid growth. To better understand the mechanism(s) involved, we studied ethanol effects on glycine-activated responses of ventral tegmental area (VTA) neurons isolated from the newborn rat, using whole cell and gramicidin perforated patch-clamp techniques. Previously we reported that 0.1\u201340 mM ethanol enhances glycine-induced responses of 35% of VTA neurons ( Ye et al. 2001 ). We now direct our attention to the inhibitory effects of ethanol observed in 45% (312 of 694) of neonatal VTA neurons. Under current-clamp conditions, 1 mM ethanol had no effect on the membrane potential of these cells, but it decreased glycine-induced membrane depolarization and the frequency of spontaneous action potentials. Under voltage-clamp conditions, 0.1\u201310 mM ethanol did not elicit a current but depressed the glycine-induced currents. The ethanol-induced inhibition of glycine current was independent of membrane potential (between \u221260 and +60 mV). Likewise, ethanol did not alter the reversal potential of the glycine-activated currents. Ethanol-mediated inhibition of glycine current depended on the glycine concentration. While ethanol strongly depressed currents activated by 30 \u03bcM glycine, it had no appreciable effect on maximal currents activated by 1 mM glycine. In the presence of ethanol (1 mM), the EC<jats:sub>50<\/jats:sub>for glycine increased from 32 \u00b1 5 to 60 \u00b1 3 \u03bcM. Thus ethanol may decrease the agonist affinity of glycine receptors. A kinetic analysis indicated that ethanol shortens the time constant of glycine current deactivation but has no effect on activation. In conclusion, by altering VTA neuronal function, ethanol-induced changes in glycine receptors may contribute to neurobehavioral manifestations of the fetal alcohol syndrome.<\/jats:p>","DOI":"10.1152\/jn.2001.86.5.2426","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T11:32:54Z","timestamp":1513855974000},"page":"2426-2434","source":"Crossref","is-referenced-by-count":17,"title":["Ethanol Inhibition of Glycine-Activated Responses in Neurons of Ventral Tegmental Area of Neonatal Rats"],"prefix":"10.1152","volume":"86","author":[{"given":"Jiang Hong","family":"Ye","sequence":"first","affiliation":[{"name":"Departments of Anesthesiology and Pharmacology and Physiology, New Jersey Medical School, Newark, New Jersey 07103-2714; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Tao","sequence":"additional","affiliation":[{"name":"Departments of Anesthesiology and Pharmacology and Physiology, New Jersey Medical 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