{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T03:39:00Z","timestamp":1773459540092,"version":"3.50.1"},"reference-count":53,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2002,8,1]]},"abstract":"<jats:p> The activity of gap junction channels between cultured and clustered carotid body glomus cells of the rat was studied with dual voltage clamping during normoxia (Po <jats:sub>2<\/jats:sub> 300 Torr) and hypoxia induced by sodium dithionite (Na<jats:sub>2<\/jats:sub>S<jats:sub>2<\/jats:sub>O<jats:sub>4<\/jats:sub>) or 100% N<jats:sub>2<\/jats:sub>. Na<jats:sub>2<\/jats:sub>S<jats:sub>2<\/jats:sub>O<jats:sub>4<\/jats:sub>reduced the saline Po <jats:sub>2<\/jats:sub> to \u223c10 Torr, whereas 100% N<jats:sub>2<\/jats:sub> reduced ambient O<jats:sub>2<\/jats:sub> to \u223c60 Torr. The following observations were made. 1) In normoxia, the intercellular macroconductance ( G <jats:sub>j<\/jats:sub> = 3.0 \u00b1 1.01 ns, mean \u00b1 SE) was changed unevenly (increased and decreased) under hypoxic conditions by either agent, although N<jats:sub>2<\/jats:sub> produced the largest changes. 2) The intercellular microconductances of the channels ( g <jats:sub>j<\/jats:sub> = 104.44 \u00b1 10.16 pS under normoxic conditions) significantly decreased in 100% N<jats:sub>2<\/jats:sub> but showed depressions and enhancements in Na<jats:sub>2<\/jats:sub>S<jats:sub>2<\/jats:sub>O<jats:sub>4<\/jats:sub>. 3) The conductance of single-junction channels (SChs), calculated as g <jats:sub>j<\/jats:sub> variance\/mean g <jats:sub>j<\/jats:sub>, yielded a mean of \u223c17.6 pS. Larger values were obtained with manual measurements of the data (\u223c34 pS). Hypoxic hypoxia (induced by 100% N<jats:sub>2<\/jats:sub>) significantly depressed the conductance of SChs when calculated from digitized records or from manual measurements. Hypoxia induced by Na<jats:sub>2<\/jats:sub>S<jats:sub>2<\/jats:sub>O<jats:sub>4<\/jats:sub>did not significantly change junctional conductance. 4) The number of intercellular channels, calculated as g <jats:sub>j<\/jats:sub>\/SCh g <jats:sub>j<\/jats:sub>, had a mean of \u223c452 (range 1 to 2,471). During N<jats:sub>2<\/jats:sub>-induced hypoxia, this number significantly decreased to \u223c84 but remained unchanged during Na<jats:sub>2<\/jats:sub>S<jats:sub>2<\/jats:sub>O<jats:sub>4<\/jats:sub>hypoxia. 5) The mean open time of junction channels varied from 4 to 30 ms in different experiments, having an overall mean of \u03bc = 11.33 \u00b1 0.33 ms. This value was significantly reduced by 100% N<jats:sub>2<\/jats:sub> but was not changed by Na<jats:sub>2<\/jats:sub>S<jats:sub>2<\/jats:sub>O<jats:sub>4<\/jats:sub>. 6) Intracellular calcium ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>), 46.2 \u00b1 4.84 nM under normoxia, significantly increased to 77.32 \u00b1 11.27 nM with Na<jats:sub>2<\/jats:sub>S<jats:sub>2<\/jats:sub>O<jats:sub>4<\/jats:sub>and to 66.39 \u00b1 11.64 nM with 100% N<jats:sub>2<\/jats:sub>. It is concluded that 100% N<jats:sub>2<\/jats:sub> uncouples glomus cells by significantly reducing intercellular macro- and microconductances. Hypoxia induced by Na<jats:sub>2<\/jats:sub>S<jats:sub>2<\/jats:sub>O<jats:sub>4<\/jats:sub>had variable effects. The coupling effects of hypoxia may depend on, or be aided by, increases in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and\/or intracellular pH changes. However, secreted transmitters and ATP plus the effects of hypoxia on second messengers and other cytoplasmic components may also play an important role in this phenomenon. <\/jats:p>","DOI":"10.1152\/jn.2002.88.2.639","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T19:55:37Z","timestamp":1513886137000},"page":"639-649","source":"Crossref","is-referenced-by-count":19,"title":["Behavior of Junction Channels Between Rat Glomus Cells During Normoxia and Hypoxia"],"prefix":"10.1152","volume":"88","author":[{"given":"Ver\u00f3nica","family":"Abudara","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Utah School of Medicine, Salt Lake City, Utah 84108-1297; and"},{"name":"Department of Physiology, Facultad de Medicina, Universidad de la Rep\u00fablica, Montevideo, Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. G.","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Utah School of Medicine, Salt Lake City, Utah 84108-1297; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Eyzaguirre","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Utah School of Medicine, Salt Lake City, Utah 84108-1297; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(96)00344-7"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-5891-0_22"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)00127-9"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)01946-0"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1007\/0-306-46825-5_33"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)02862-1"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1973.sp010410"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.77.1.174"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1994.sp020143"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.00719.x"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.2.C533"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.8.8338"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-08-02835.2000"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2000.279.1.L36"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1991.tb02127.x"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199807000-00012"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5687(99)00020-1"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1982.sp014394"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1993.70.4.1378"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-6408(1999)24:1\/2<123::AID-DVG12>3.0.CO;2-H"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1983.sp014842"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-02-00520.1999"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)90969-3"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)90326-Q"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(20000228)418:1<33::AID-CNE3>3.0.CO;2-J"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.6.1349"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2001.280.2.L221"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-5891-0_4"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1007\/BF00386175"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(93)81242-6"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1159\/000127130"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)90810-A"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1995.269.4.H1481"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374302"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1007\/BF01870342"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(86)91056-5"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(86)90100-9"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)91453-Y"},{"key":"B45","first-page":"365","volume":"26","author":"Pang L","year":"1993","journal-title":"Biol Res"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1990.tb03137.x"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.04-10-02477.1984"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(85)90901-1"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.4710"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1996.tb01312.x"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)90951-Q"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.279.2.C295"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.47.030185.001433"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(99)00373-9"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1988.254.1.H170"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(89)90030-X"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.95.6.1061"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)01249-9"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(95)00158-M"}],"container-title":["Journal of Neurophysiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jn.2002.88.2.639","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T04:32:16Z","timestamp":1568003536000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jn.2002.88.2.639"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,8,1]]},"references-count":53,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,8,1]]}},"alternative-id":["10.1152\/jn.2002.88.2.639"],"URL":"https:\/\/doi.org\/10.1152\/jn.2002.88.2.639","relation":{},"ISSN":["0022-3077","1522-1598"],"issn-type":[{"value":"0022-3077","type":"print"},{"value":"1522-1598","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,8,1]]}}}