{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T21:38:03Z","timestamp":1767908283183,"version":"3.49.0"},"reference-count":55,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2002,10,1]]},"abstract":"<jats:p>The hypothalamus influences a number of autonomic functions. The activity of hypothalamic neurons is modulated in part by release of the inhibitory neurotransmitter GABA onto these neurons. GABA<jats:sub>A<\/jats:sub>receptors are formed from a number of distinct subunits, designated \u03b1, \u03b2, \u03b3, \u03b4, \u03b5, and \u03b8, many of which have multiple isoforms. Little data exist, however, on the functional characteristics of the GABA<jats:sub>A<\/jats:sub>receptors present on hypothalamic neurons. To gain insight into which GABA<jats:sub>A<\/jats:sub>receptor subunits are functionally expressed in the hypothalamus, we used an array of pharmacologic assessments. Whole cell recordings were made from thin hypothalamic slices obtained from 1- to 14-day-old rats. GABA<jats:sub>A<\/jats:sub>receptor-mediated currents were detected in all neurons tested and had an average EC<jats:sub>50<\/jats:sub>of 20 \u00b1 1.6 \u03bcM. Hypothalamic GABA<jats:sub>A<\/jats:sub>receptors were modulated by diazepam (EC<jats:sub>50<\/jats:sub>= 0.060 \u03bcM), zolpidem (EC<jats:sub>50<\/jats:sub>= 0.19 \u03bcM), loreclezole (EC<jats:sub>50<\/jats:sub>= 4.4 \u03bcM), methyl-6,7-dimethoxy-4-ethyl-\u03b2-carboline (EC<jats:sub>50<\/jats:sub>= 7.7 \u03bcM), and 5\u03b1-pregnan-3\u03b1-hydroxy-20-one (3\u03b1-OH-DHP). Conversely, these receptors were inhibited by Zn<jats:sup>2+<\/jats:sup>(IC<jats:sub>50<\/jats:sub>= 70.5 \u03bcM), dehydroepiandrosterone sulfate (IC<jats:sub>50<\/jats:sub>= 16.7 \u03bcM), and picrotoxin (IC<jats:sub>50<\/jats:sub>= 2.6 \u03bcM). The \u03b14\/6-selective antagonist furosemide (10\u20131,000 \u03bcM) was ineffective in all hypothalamic neurons tested. The results of our pharmacological analysis suggest that hypothalamic neurons express functional GABA<jats:sub>A<\/jats:sub>receptor subtypes that incorporate \u03b11 and\/or \u03b12 subunits, \u03b22 and\/or \u03b23 subunits, and the \u03b32 subunit. Our results suggest receptors expressing \u03b13\u2013\u03b16, \u03b21, \u03b31, and \u03b4, if present, represent a minor component of functional hypothalamic GABA<jats:sub>A<\/jats:sub>receptors.<\/jats:p>","DOI":"10.1152\/jn.2002.88.4.1655","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T21:14:52Z","timestamp":1513890892000},"page":"1655-1663","source":"Crossref","is-referenced-by-count":11,"title":["Functional Characterization of GABA<sub>A<\/sub>Receptors in Neonatal Hypothalamic Brain Slice"],"prefix":"10.1152","volume":"88","author":[{"given":"Ren-Qi","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, Fort Worth, Texas 76107"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glenn H.","family":"Dillon","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Neuroscience, University of North Texas 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