{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:30:31Z","timestamp":1790980231077,"version":"4.1.0"},"reference-count":46,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:p>\n                    The function and biophysical properties of low threshold Kv1 current in control of membrane resonance, subthreshold oscillations, and bursting in mesencephalic V neurons (Mes V) were examined in rat brain stem slices (P8\u2013P12) using whole cell current and voltage patch-clamp methods. \u03b1-dendrotoxin application, a toxin with high specificity for Kv1.1, 1.2, and 1.6 channels, showed the presence of a low-threshold K\n                    <jats:sup>+<\/jats:sup>\n                    current that activated rapidly around \u221250 mV and was relatively noninactivating over a 1-s period and had a V\n                    <jats:sub>1\/2<\/jats:sub>\n                    max of \u221236.2 mV. Other toxins, specific for individual channels containing either Kv 1.1, 1.2, or 1.3 \u03b1-subunits, were applied individually, or in combination, and showed that Kv1 channels are heteromeric, composed of combinations of subunits. In current-clamp mode, toxin application transformed the high-frequency resonant properties of the membrane into a low-pass filter and concomitantly reduced the frequency of the subthreshold membrane oscillations. During this period, rhythmical bursting was transformed into low-frequency tonic discharge. Interestingly, in a subset of neurons that did not show bursting, low doses of \u03b1-dendrotoxin (\u03b1-DTX) sufficient to block 50% of the low threshold Kv1 channels induced bursting and increased the resonant peak impedance and subthreshold oscillations, which was replicated with computer simulation. This suggests that a critical balance between inward and outward currents is necessary for bursting. This was replicated with computer simulation. Single cell RT-PCR and immunohistochemical methods confirmed the presence of Kv1.1, 1.2, and 1.6 \u03b1-subunits in Mes V neurons. These data indicate that low threshold Kv1 channels are responsible for membrane resonance, contribute to subthreshold oscillations, and are critical for burst generation.\n                  <\/jats:p>","DOI":"10.1152\/jn.91053.2008","type":"journal-article","created":{"date-parts":[[2009,1,14]],"date-time":"2009-01-14T22:36:29Z","timestamp":1231972589000},"page":"1407-1418","source":"Crossref","is-referenced-by-count":33,"title":["Participation of Kv1 Channels in Control of Membrane Excitability and Burst Generation in Mesencephalic V 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