{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T06:11:28Z","timestamp":1787638288799,"version":"build-2736575974"},"reference-count":37,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:p> The regulation of the benzamil (Bz)-insensitive salt taste receptor was investigated by intracellular Ca<jats:sup>2+<\/jats:sup> ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>), protein kinase C (PKC), and the Ca<jats:sup>2+<\/jats:sup>-dependent serine-threonine phosphatase, calcineurin (PP2B), by monitoring chorda tympani taste nerve responses to 0.1 M NaCl solutions containing Bz (5 \u00d7 10<jats:sup>\u22126<\/jats:sup> M) and resiniferatoxin (RTX; 0\u201310 \u00d7 10<jats:sup>\u22126<\/jats:sup> M) in Sprague\u2013Dawley rats and in wild-type (WT) and transient receptor potential vanilloid-1 knockout (TRPV1 KO) mice. In rats and WT mice, RTX increased the NaCl + Bz chorda tympani responses between 0.25 \u00d7 10<jats:sup>\u22126<\/jats:sup> and 1 \u00d7 10<jats:sup>\u22126<\/jats:sup> M and inhibited the responses above 1 \u00d7 10<jats:sup>\u22126<\/jats:sup> M. Decreasing taste receptor cell (TRC) [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> with BAPTA loading, activation of PKC with 4\u03b1-phorbol-12,13-didecanoate (PMA), or inhibition of PP2B by cyclosporin A or FK-506, enhanced the magnitude of the Bz-insensitive NaCl chorda tympani responses in the presence of RTX and either minimized or completely eliminated the decrease in the chorda tympani response &gt;1 \u00d7 10<jats:sup>\u22126<\/jats:sup> M RTX. In contrast, increasing TRC [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> with ionomycin inhibited Bz-insensitive NaCl chorda tympani responses in the presence of RTX. No effect of the cited modulators was observed on the chorda tympani responses in WT mice and rats in the presence of TRPV1 blocker SB-366791 (1 \u00d7 10<jats:sup>\u22126<\/jats:sup> M) or in TRPV1 KO mice. <jats:sup>32<\/jats:sup>P-labeling demonstrated direct phosphorylation of TRPV1 or TRPV1t in anterior lingual epithelium by PMA, cyclosporin A, or FK-506. PMA also enhanced the RTX-sensitive unilateral apical Na<jats:sup>+<\/jats:sup> flux in polarized fungiform TRC in vitro. We conclude that TRPV1 or its variant TRPV1t is phosphorylated and dephosphorylated by PKC and PP2B, respectively, and either sensitizes or desensitizes the Bz-insensitive NaCl chorda tympani responses to RTX stimulation. <\/jats:p>","DOI":"10.1152\/jn.91301.2008","type":"journal-article","created":{"date-parts":[[2009,6,25]],"date-time":"2009-06-25T01:52:47Z","timestamp":1245894767000},"page":"1591-1605","source":"Crossref","is-referenced-by-count":23,"title":["Regulation of the Benzamil-Insensitive Salt Taste Receptor by Intracellular Ca<sup>2+<\/sup>, Protein Kinase C, and Calcineurin"],"prefix":"10.1152","volume":"102","author":[{"given":"Vijay","family":"Lyall","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tam-Hao T.","family":"Phan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shobha","family":"Mummalaneni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Melone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunila","family":"Mahavadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karnam S.","family":"Murthy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"DeSimone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.111534"},{"key":"R2","doi-asserted-by":"crossref","unstructured":"Bhave G, Hu HJ, Glauner KS, Zhu W, Wang H, Brasier DJ, Oxford GS, Gereau RW 4th. 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