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Quantitative real-time RT-PCR confirmed a largely overlapping gene expression profile subsequent to EP2 and EP4 agonist treatment, with 65 significantly regulated genes identified overall, 5 being specific for the EP2 agonist and 6 specific for the EP4 agonist. We found predicted functional cAMP-response elements in promoter regions of a large fraction of the predominantly upregulated genes, which suggests that the cAMP signaling pathway is the most important intracellular signaling pathway for these agonists in these cells. Several target genes were identified that, as part of complex regulatory networks, are implicated in tissue remodeling processes and osmoregulation (e.g., AREG, LOXL3, BMP2, AQP3) and thus may help elucidate the mechanism of action of these IOP-lowering drugs involving the uveo-scleral outflow path.<\/jats:p>","DOI":"10.1152\/physiolgenomics.00012.2010","type":"journal-article","created":{"date-parts":[[2010,6,16]],"date-time":"2010-06-16T01:36:10Z","timestamp":1276652170000},"page":"348-360","source":"Crossref","is-referenced-by-count":10,"title":["Prostaglandin E<sub>2<\/sub>receptor subtype EP2- and EP4-regulated gene expression profiling in human ciliary smooth muscle cells"],"prefix":"10.1152","volume":"42","author":[{"given":"Armin","family":"Reitmair","sequence":"first","affiliation":[{"name":"Department of Biological Sciences and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nils W. 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