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Currently, baseline data describing genome-wide coding and long noncoding gene expression profiles in the healthy colon specific to tissue type and location are lacking. Therefore, colonic mucosal biopsies from 10 healthy participants who were enrolled in a clinical study to evaluate effects of lignan supplementation on gut resiliency were used to characterize the site-specific global gene expression signatures associated with stromal vs. epithelial cells in the sigmoid colon and rectum. Using RNA-seq, we demonstrate that tissue type and location patterns of gene expression and upstream regulatory pathways are distinct. For example, consistent with a key role of stroma in the crypt niche, mRNAs associated with immunoregulatory and inflammatory processes (i.e., CXCL14, ANTXR1), smooth muscle contraction (CALD1), proliferation and apoptosis (GLP2R, IGFBP3), and modulation of extracellular matrix (MMP2, COL3A1, MFAP4) were all highly expressed in the stroma. In comparison, HOX genes (HOXA3, HOXD9, HOXD10, HOXD11, and HOXD-AS2, a HOXD cluster antisense RNA 2), and WNT5B expression were also significantly higher in sigmoid colon compared with the rectum. These findings provide strong impetus for considering colorectal tissue subtypes and location in future observational studies and clinical trials designed to evaluate the effects of exposures on colonic health. <\/jats:p>","DOI":"10.1152\/physiolgenomics.00023.2016","type":"journal-article","created":{"date-parts":[[2016,7,12]],"date-time":"2016-07-12T03:39:02Z","timestamp":1468294742000},"page":"651-659","source":"Crossref","is-referenced-by-count":13,"title":["Comprehensive site-specific whole genome profiling of stromal and epithelial colonic gene signatures in human sigmoid colon and rectal tissue"],"prefix":"10.1152","volume":"48","author":[{"given":"Jason M.","family":"Knight","sequence":"first","affiliation":[{"name":"Department of Electrical Engineering, Texas A&amp;M University, College Station, Texas;"},{"name":"Center for Translational Environmental Health Research, Texas A&amp;M University, College Station, Texas;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eunji","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering, Texas A&amp;M University, College Station, Texas;"},{"name":"Center for Translational Environmental Health Research, Texas A&amp;M University, College Station, Texas;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"Ivanov","sequence":"additional","affiliation":[{"name":"Department of Veterinary Physiology &amp; Pharmacology, Texas A&amp;M University, College Station, Texas;"},{"name":"Center for Translational Environmental Health Research, Texas A&amp;M University, College Station, Texas;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurie A.","family":"Davidson","sequence":"additional","affiliation":[{"name":"Department of Nutrition &amp; Food Science, Texas A&amp;M University, College Station, Texas;"},{"name":"Center for Translational Environmental Health Research, Texas A&amp;M University, College Station, Texas;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer S.","family":"Goldsby","sequence":"additional","affiliation":[{"name":"Department of Nutrition &amp; Food Science, Texas A&amp;M University, College Station, Texas;"},{"name":"Center for Translational Environmental Health Research, Texas A&amp;M University, College Station, Texas;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meredith A. 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