{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:41:19Z","timestamp":1762101679105,"version":"3.41.0"},"reference-count":29,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiological Genomics"],"published-print":{"date-parts":[[2002,2,28]]},"abstract":"<jats:p>Parotid gland acinar cells undergo marked hypertrophy and hyperplasia upon systemic exposure to the \u03b2-adrenergic agonist, isoproterenol. This glandular enlargement is accompanied by substantial cellular changes including DNA synthesis, an increase in glandular protein synthesis, and differential changes in RNA transcription. To gain a more detailed understanding of the underlying changes induced by isoproterenol, we have examined the parotid gland gene expression profile of mice up to 24 h post-isoproterenol injection using high-density oligonucleotide arrays. Depending upon the exposure time, between 22 and 48 of the \u223c6,500 mouse genes and expressed sequence tags (ESTs) analyzed displayed significant changes in expression patterns. Genes that were previously shown to be repressed (\u03b1-amylase) or activated (proline-rich proteins) following isoproterenol exposure were found to be similarly affected in this experiment, validating this technique. This study demonstrates that the oligonucleotide array technology is a useful tool for examining isoproterenol-induced salivary gland gene expression changes. Using this as a starting point, we can begin to dissect the specific pathways involved in mediating isoproterenol action within the parotid gland.<\/jats:p>","DOI":"10.1152\/physiolgenomics.00039.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:56:30Z","timestamp":1425416190000},"page":"107-114","source":"Crossref","is-referenced-by-count":21,"title":["Analysis of isoproterenol-induced changes in parotid gland gene expression"],"prefix":"10.1152","volume":"8","author":[{"given":"Kelly G.","family":"Ten Hagen","sequence":"first","affiliation":[{"name":"Center for Oral Biology, Aab Institute for Biomedical Sciences, University of Rochester, Rochester, New York 14642"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marlene M.","family":"Balys","sequence":"additional","affiliation":[{"name":"Center for Oral Biology, Aab Institute for Biomedical Sciences, University of Rochester, Rochester, New York 14642"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lawrence A.","family":"Tabak","sequence":"additional","affiliation":[{"name":"Center for Oral Biology, Aab Institute for Biomedical Sciences, University of Rochester, Rochester, New York 14642"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James E.","family":"Melvin","sequence":"additional","affiliation":[{"name":"Center for Oral Biology, Aab Institute for Biomedical Sciences, University of Rochester, Rochester, New York 14642"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"doi-asserted-by":"crossref","unstructured":"Ann DK, Clements S, Johnstone EM, and Carlson DM.Induction of tissue-specific proline-rich protein multigene families in rat and mouse parotid glands by isoproterenol. 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