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The nematode counterparts of the human ATP-binding cassette half-transporters, the enzymes alkyldihydroxyacetonephosphate synthase and \u0394<jats:sup>3,5<\/jats:sup>-\u0394<jats:sup>2,4<\/jats:sup>-dienoyl-CoA isomerase, the receptors for peroxisomal membrane and matrix proteins (Pex19p and Pex5p), and components of the docking and translocation machineries for matrix proteins (Pex13p and Pex12p) are essential for the development of C. elegans. Unexpectedly, RNAi silencing of the acyl-CoA synthetase-mediated activation of fatty acids, the \u03b1- and \u03b2-oxidation of fatty acids, the intraperoxisomal decomposition of hydrogen peroxide, and the peroxins Pex1p, Pex2p, and Pex6p had no apparent effect on C. elegans development. The described analysis of functional gene knockouts through RNAi provides a basis for the use of C. elegans as a valuable model system with which to study the molecular and physiological defects underlying the human peroxisomal disorders.<\/jats:p>","DOI":"10.1152\/physiolgenomics.00044.2002","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:50:52Z","timestamp":1425415852000},"page":"79-91","source":"Crossref","is-referenced-by-count":42,"title":["RNA interference of peroxisome-related genes in<i>C. elegans<\/i>: a new model for human peroxisomal disorders"],"prefix":"10.1152","volume":"10","author":[{"given":"Oleh I.","family":"Petriv","sequence":"first","affiliation":[{"name":"Department of Cell Biology, University of Alberta, Edmonton T6G 2H7"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David B.","family":"Pilgrim","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Alberta, 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