{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:46:21Z","timestamp":1790621181199,"version":"4.1.0"},"reference-count":37,"publisher":"American Physiological Society","issue":"7","funder":[{"name":"Ito Foundation"}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["Physiological Genomics"],"published-print":{"date-parts":[[2021,7,1]]},"abstract":"<jats:p> We investigated changes in rumen fermentation, peripheral blood metabolites and hormones, and hepatic transcriptomic dynamics in Holstein cows with and those without subacute ruminal acidosis (SARA) during the periparturient period. Sixteen multiparous Holstein cows were categorized in the SARA ( n = 8) or non-SARA ( n = 8) groups depending on whether they developed SARA during the 2\u2009wk after parturition. Reticulo-ruminal pH was measured continuously throughout the study. Rumen fluid, blood, and liver tissue samples were collected at 3\u2009wk prepartum and 2 and 6\u2009wk postpartum, with an additional blood sample collected at 0 and 4\u2009wk postpartum. The 1-h mean pH was depressed postpartum in both groups, whereas depression was more severe in the SARA group simultaneously with significantly longer duration of time (for pH &lt;5.6 and 5.8). Significant expression of differentially expressed genes in liver tissue (DEGs; false discovery rate corrected P &lt; 0.1) were identified only in the non-SARA group and were further analyzed by Ingenuity Pathway Analysis software. Among the top expressed DEGs, the hepatic genes encoding lipid and cholesterol secretion (APOA1, APOA4, and G0S2) and gluconeogenesis (PC, G6PC, and PCK1) were upregulated postpartum. In silico analysis revealed the significant postpartum activation of upstream regulators, such as INSR, PPARG, and PPARGC1A. These results suggested that hepatic transcriptomic responsiveness to postpartum metabolic load and hormones were likely discouraged in cows with SARA when compared with the significant activation of genes and signaling pathways for adequate metabolic adaption to postpartum high-grain diet feeding in Holstein cows without SARA. <\/jats:p>","DOI":"10.1152\/physiolgenomics.00048.2021","type":"journal-article","created":{"date-parts":[[2021,6,7]],"date-time":"2021-06-07T16:06:02Z","timestamp":1623081962000},"page":"285-294","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Liver transcriptome response to periparturient hormonal and metabolic changes depends on the postpartum occurrence of subacute ruminal acidosis in Holstein cows"],"prefix":"10.1152","volume":"53","author":[{"given":"Yoshiyuki","family":"Tsuchiya","sequence":"first","affiliation":[{"name":"Graduate School of Veterinary Sciences, Iwate University, 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