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We used RNA-Seq analysis to examine the global profile of gene expression in a rat serotonergic cell line (RN46A) after exposure to these two mood stabilizer drugs. Numerous genes were differentially regulated in response to VPA (log\n                    <jats:sub>2<\/jats:sub>\n                    fold change \u2265 1.0; i.e., odds ratio of \u22652, at false discovery rate &lt;5%), but only two genes ( Dynlrb2 and Cdyl2) showed significant differential regulation after exposure of the cells to lithium, with the same analysis criteria. Both of these genes were also regulated by VPA. Many of the differentially expressed genes had functions of potential relevance to mood disorders or their treatment, such as several serpin family genes (including neuroserpin), Nts (neurotensin), Maob (monoamine oxidase B), and Ap2b1, which is important for synaptic vesicle function. Pathway analysis revealed significant enrichment of Gene Ontology terms such as extracellular matrix remodeling, cell adhesion, and chemotaxis. This study in a cell line derived from the raphe nucleus has identified a range of genes and pathways that provide novel insights into potential therapeutic actions of the commonly used mood stabilizer drugs.\n                  <\/jats:p>","DOI":"10.1152\/physiolgenomics.00069.2018","type":"journal-article","created":{"date-parts":[[2018,12,21]],"date-time":"2018-12-21T12:07:21Z","timestamp":1545394041000},"page":"43-50","source":"Crossref","is-referenced-by-count":23,"title":["Gene expression effects of lithium and valproic acid in a serotonergic cell line"],"prefix":"10.1152","volume":"51","author":[{"given":"Diana","family":"Balasubramanian","sequence":"first","affiliation":[{"name":"Carney Centre for Pharmacogenomics, Department of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John F.","family":"Pearson","sequence":"additional","affiliation":[{"name":"Carney Centre for Pharmacogenomics, Department of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand"},{"name":"Biostatistics and Computational Biology Unit, University of Otago, Christchurch, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6445-8526","authenticated-orcid":false,"given":"Martin A.","family":"Kennedy","sequence":"additional","affiliation":[{"name":"Carney Centre for Pharmacogenomics, Department of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1186\/2049-9256-1-16"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-10-r106"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2015.06.018"},{"key":"B4","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","volume":"57","author":"Benjamini Y","year":"1995","journal-title":"J R Stat Soc B"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-13-S16-S13"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.3109\/10409238.2011.619164"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1186\/1756-8935-6-11"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.02.006"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1177\/2045125312471963"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/479364"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1999.720879.x"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1017\/S1461145705005717"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1097\/YPG.0b013e3282fb0051"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD003196.pub2"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1038\/sj.npp.1300288"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4306"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1077(200003)15:2<113::AID-HUP150>3.0.CO;2-W"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1038\/43894"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3039"},{"key":"B22","doi-asserted-by":"crossref","first-page":"13983","DOI":"10.1016\/S0021-9258(18)68172-4","volume":"263","author":"Dobner PR","year":"1988","journal-title":"J Biol Chem"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1995.1205"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.21273"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-013-9907-x"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R113.451211"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2016.00340"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M802655200"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1017\/S1461145708009231"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.2174\/187152012802650228"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD009183.pub2"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1038\/tpj.2009.33"},{"key":"B33","unstructured":"Goodwin FK, Jamison KR. 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