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Peak V\u0307o<jats:sub>2<\/jats:sub> (measured by cardiopulmonary exercise testing) and total RNA from PBMCs were obtained before and after 18 wk of running endurance training (3 times\/wk, 60 min). Total RNA was used for whole genome expression analysis using Affymetrix GeneChip Human Gene 1.0 ST. Data were normalized by the robust multiarray average algorithm. Principal component analysis was used to perform correlations between baseline gene expression and V\u0307o<jats:sub>2peak<\/jats:sub>. A set of 211 transcripts was differentially expressed (ANOVA, P &lt; 0.05 and fold change &gt; 1.3). Functional enrichment analysis revealed that transcripts were mainly related to immune function, cell cycle processes, development, and growth. Baseline expression of 98 and 53 transcripts was associated with the absolute and relative V\u0307o<jats:sub>2peak<\/jats:sub> response, respectively, with a strong correlation ( r &gt; 0.75, P &lt; 0.01), and this panel was able to classify the 13 individuals according to their potential to improve oxygen uptake. A subset of 10 transcripts represented these signatures to a similar extent. PBMCs reveal a transcriptional signature responsive to endurance training. Additionally, a baseline transcriptional signature was associated with changes in V\u0307o<jats:sub>2peak<\/jats:sub>. Results might illustrate the possibility of obtaining molecular classifiers of endurance capacity changes through a minimally invasive blood sampling procedure. <\/jats:p>","DOI":"10.1152\/physiolgenomics.00072.2014","type":"journal-article","created":{"date-parts":[[2014,12,2]],"date-time":"2014-12-02T23:55:09Z","timestamp":1417564509000},"page":"13-23","source":"Crossref","is-referenced-by-count":34,"title":["PBMCs express a transcriptome signature predictor of oxygen uptake responsiveness to endurance exercise training in men"],"prefix":"10.1152","volume":"47","author":[{"given":"Rodrigo Gon\u00e7alves","family":"Dias","sequence":"first","affiliation":[{"name":"Laboratory of Genetics and Molecular Cardiology, Heart Institute (InCor) University of S\u00e3o Paulo Medical School, S\u00e3o Paulo, Brazil; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle Sabrina Moreira","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics 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