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Dstn<jats:sup> corn1<\/jats:sup> mice exhibit an actin dynamics defect in the corneal epithelial cells, offering an in vivo model to investigate cellular mechanisms affected by the Dstn mutation and resultant actin dynamics abnormalities. To examine the effect of the Dstn<jats:sup> corn1<\/jats:sup> mutation on the gene expression profile, we performed a microarray analysis using the cornea from Dstn<jats:sup> corn1<\/jats:sup> and wild-type mice. A dramatic alteration of the gene expression profile was observed in the Dstn<jats:sup> corn1<\/jats:sup> cornea, with 1,226 annotated genes differentially expressed. Functional annotation of these genes revealed that the most significantly enriched functional categories are associated with actin and\/or cytoskeleton. Among genes that belong to these categories, a considerable number of serum response factor target genes were found, indicating the possible existence of an actin-SRF pathway of transcriptional regulation in vivo. A comparative study using an allelic mutant strain with milder corneal phenotypes suggested that the level of filamentous actin may correlate with the level of gene expression changes. Our study shows that Dstn mutations and resultant actin dynamics abnormalities have a strong impact on the gene expression profile in vivo. <\/jats:p>","DOI":"10.1152\/physiolgenomics.00285.2007","type":"journal-article","created":{"date-parts":[[2008,4,1]],"date-time":"2008-04-01T23:24:39Z","timestamp":1207092279000},"page":"9-21","source":"Crossref","is-referenced-by-count":27,"title":["Effect of destrin mutations on the gene expression profile in vivo"],"prefix":"10.1152","volume":"34","author":[{"given":"Angela M.","family":"Verdoni","sequence":"first","affiliation":[{"name":"Department of Medical Genetics, University of Wisconsin-Madison, Madison, Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natsuyo","family":"Aoyama","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, University of Wisconsin-Madison, Madison, Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiro","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, University of Wisconsin-Madison, Madison, Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakae","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, University of Wisconsin-Madison, Madison, Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","unstructured":"Affymetrix I. 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