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In all transgenic muscles, HSP72 was expressed at higher levels compared with transgene-negative controls, where HSP72 was below the level of detection. At the same time, HSC73 levels were downregulated in all transgenic muscle types. Shipment-related stress caused an elevation in the levels of HSP72 in all muscles for 1 wk after arrival of the animals. We also found that, although no statistical differences in response to intermittent fatiguing stimulation in the contractile properties of intact transgene-positive muscles compared with their transgene-negative counterparts were observed, the response of intact transgene-positive EDL muscles to caffeine was enhanced. These findings demonstrate that elevated HSP72 does not protect EDL, SOL, or DPH muscles from the effects of intermittent fatiguing stimulation. However, HSP72 may influence the excitation-contraction coupling (ECC) process, either directly or indirectly, in EDL muscle. If the effects on ECC were indirect, then these results would suggest that manipulation of a specific gene might cause functional effects that seem independent of the manipulated gene\/protein.<\/jats:p>","DOI":"10.1152\/physiolgenomics.2000.4.1.25","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T22:53:16Z","timestamp":1514069596000},"page":"25-33","source":"Crossref","is-referenced-by-count":22,"title":["Functional properties of skeletal muscle from transgenic animals with upregulated heat shock protein 70"],"prefix":"10.1152","volume":"4","author":[{"given":"THOMAS M.","family":"NOSEK","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4963"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MARCO A. 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