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The present study was designed to determine the characteristics of HIF-1\u03b1 cDNA cloned from the rat kidney and the expression profile of HIF-1\u03b1 in different kidney regions and to explore the mechanism activating or regulating HIF-1\u03b1 expression in renal medullary cells. A 3,718-bp HIF-1\u03b1 cDNA from the rat kidney was first cloned and sequenced using RT-PCR and TA cloning technique. It was found that 823 amino acids deduced from this renal HIF-1\u03b1 cDNA had 99%, 96%, and 90% identity with rat, mouse, or human HIF-1\u03b1 deposited in GenBank, respectively. The 3\u2032-untranslated region of HIF-1\u03b1 mRNA from the rat kidney contained seven AUUUA instability elements, five of which were found to be conserved among rat, mouse, and human HIF-1\u03b1. Northern blot analyses demonstrated a corticomedullary gradient of HIF-1\u03b1 mRNA expression in the kidney, with the greatest abundance in the renal inner medulla. Western blot analyses also detected a higher HIF-1\u03b1 protein level in the nuclear extracts from the renal medulla than the renal cortex. A classic loop diuretic, furosemide (10 mg\/kg ip), markedly increased renal medullary Po<jats:sub>2<\/jats:sub>levels from 22.5 to 52.2 mmHg, which was accompanied by a significant reduction of HIF-1\u03b1 transcripts in renal medullary tissue. In in vitro experiments, low Po<jats:sub>2<\/jats:sub>, but not elevated osmolarity, was found to significantly increase HIF-1\u03b1 mRNA in renal medullary interstitial cells and inner medullary collecting duct cells. These results indicate that HIF-1\u03b1 is more abundantly expressed in the renal medulla compared with the renal cortex. Increased abundance of HIF-1\u03b1 mRNA in the renal medulla may represent an adaptive response of renal medullary cells to low Po<jats:sub>2<\/jats:sub>.<\/jats:p>","DOI":"10.1152\/physiolgenomics.2001.6.3.159","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T23:53:49Z","timestamp":1514073229000},"page":"159-168","source":"Crossref","is-referenced-by-count":50,"title":["Oxygen-dependent expression of hypoxia-inducible factor-1\u03b1 in renal medullary cells of rats"],"prefix":"10.1152","volume":"6","author":[{"given":"AI-PING","family":"ZOU","sequence":"first","affiliation":[{"name":"Departments of Physiology and Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZHI-ZHANG","family":"YANG","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PIN-LAN","family":"LI","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"JR.","given":"ALLEN W.","family":"COWLEY","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115316"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1994.267.6.F1059"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1994.267.6.F1063"},{"key":"R4","unstructured":"Conrad KP and Dunn MJ.Renal prostaglandins and other eicosanoids. 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