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To define gene regulatory programs of valvulogenesis relative to osteoblast progenitors, we undertook Affymetrix gene expression profiling analysis of murine embryonic day (E)12.5 AV endocardial cushions compared with E17.5 AV valves (mitral and tricuspid) and with preosteoblast MC3T3-E1 (subclone4) cells. Overall, MC3T3 cells were significantly more similar to E17.5 valves than to E12.5 cushions, supporting the hypothesis that valve maturation involves the expression of many genes also expressed in osteoblasts. Several transcription factors characteristic of mesenchymal and osteoblast precursor cells, including Twist1, are predominant in E12.5 cushion. Valve maturation is characterized by differential regulation of matrix metalloproteinases and their inhibitors as well as complex collagen gene expression. Among the most highly enriched genes during valvulogenesis were members of the small leucine-rich proteoglycan (SLRP) family including Asporin, a known negative regulator of osteoblast differentiation and mineralization. Together, these data support shared gene expression profiles of the developing valves and osteoblast bone precursor cells in normal valve development and homeostasis with potential functions in calcific valve disease.<\/jats:p>","DOI":"10.1152\/physiolgenomics.90212.2008","type":"journal-article","created":{"date-parts":[[2008,7,9]],"date-time":"2008-07-09T05:13:47Z","timestamp":1215580427000},"page":"75-85","source":"Crossref","is-referenced-by-count":62,"title":["Shared gene expression profiles in developing heart valves and osteoblast progenitor cells"],"prefix":"10.1152","volume":"35","author":[{"given":"Santanu","family":"Chakraborty","sequence":"first","affiliation":[{"name":"Division of Molecular Cardiovascular Biology, Cincinnati Children's Medical Center, Cincinnati, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Cheek","sequence":"additional","affiliation":[{"name":"Division of Molecular Cardiovascular Biology, Cincinnati Children's 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