{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:36:38Z","timestamp":1761863798876},"reference-count":42,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[1998,10,1]]},"abstract":"<jats:p>Miyamoto, Takenori, Rie Fujiyama, Yukio Okada, and Toshihide Sato. Sour transduction involves activation of NPPB-sensitive conductance in mouse taste cells. J. Neurophysiol. 80: 1852\u20131859, 1998. We examined the sour taste transduction mechanism in the mouse by applying whole cell patch-clamp technique to nondissociated taste cells from the fungiform papillae. Localized stimulation with 0.5 M NaCl and 25 mM citric acid (pH 3.0) of the apical membrane enabled us to obtain responses from single taste cells under a quasi-natural condition. Of 28 taste cells examined, 11 cells (39%) responded to 0.5 M NaCl alone and 2 cells (7%) responded to 25 mM citric acid alone, indicating the presence of salty- and sour-specific taste cells. Ten cells (36%) responded to both NaCl and citric acid and 5 cells (18%) responded to neither salt nor citric acid. Amiloride reversibly suppressed NaCl-induced responses in mouse taste cells but not citric acid-induced responses. On the other hand, a Cl<jats:sup>\u2212<\/jats:sup>channel blocker, 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB), reversibly suppressed all the citric-acid-induced responses. Most of the NaCl-induced current responses displayed an inwardly rectifying property, whereas all the citric-acid-induced responses displayed an outwardly rectifying property. The reversal potential for NPPB-sensitive component in citric-acid-induced current responses was \u22122 \u00b1 7 mV (mean \u00b1 SE, n = 4), which was close to the equilibrium potential of Cl<jats:sup>\u2212<\/jats:sup>( E<jats:sub>Cl<\/jats:sub>), whereas the reversal potential for NPPB-insensitive component was 34 \u00b1 8 mV ( n = 4). The reversal potential of citric-acid-induced current responses (19 \u00b1 8 mV, n = 4) was mostly present at the middle point between reversal potentials of NPPB-sensitive and -insensitive current components. In some taste cells, an inorganic cation channel blocker, Cd<jats:sup>2+<\/jats:sup>, suppressed citric-acid-induced responses, but an inorganic stretch-activated cation channel blocker, Gd<jats:sup>3+<\/jats:sup>, did not affect these responses. These results suggest that salt- and acid-induced responses were mediated by differential transduction mechanisms in mouse taste cells and that NPPB-sensitive Cl<jats:sup>\u2212<\/jats:sup>channels play a more important role to sour taste transduction rather than amiloride-sensitive Na<jats:sup>+<\/jats:sup>channels. However, the fact that the reversal potentials of citric-acid-induced responses had more positive than E<jats:sub>Cl<\/jats:sub>suggests that Ca<jats:sup>2+<\/jats:sup>or H<jats:sup>+<\/jats:sup>permeable and poorly selective cation channels, which should be amiloride insensitive, may be activated by citric acid.<\/jats:p>","DOI":"10.1152\/jn.1998.80.4.1852","type":"journal-article","created":{"date-parts":[[2017,12,25]],"date-time":"2017-12-25T04:40:17Z","timestamp":1514176817000},"page":"1852-1859","source":"Crossref","is-referenced-by-count":43,"title":["Sour Transduction Involves Activation of NPPB-Sensitive Conductance in Mouse Taste Cells"],"prefix":"10.1152","volume":"80","author":[{"given":"Takenori","family":"Miyamoto","sequence":"first","affiliation":[{"name":"Department of Physiology, Nagasaki University School of Dentistry, Nagasaki 852-8588, Japan"}]},{"given":"Rie","family":"Fujiyama","sequence":"additional","affiliation":[{"name":"Department of Physiology, Nagasaki University School of Dentistry, Nagasaki 852-8588, Japan"}]},{"given":"Yukio","family":"Okada","sequence":"additional","affiliation":[{"name":"Department of Physiology, Nagasaki University School of Dentistry, Nagasaki 852-8588, Japan"}]},{"given":"Toshihide","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Physiology, Nagasaki University School of Dentistry, Nagasaki 852-8588, Japan"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01871362"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.99.4.591"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.5.C1295"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.107.4.545"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)00974-8"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(94)90221-6"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.100.5.803"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90208-9"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1093\/chemse\/13.1.89"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1007\/BF00609733"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1007\/BF00232681"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)91085-U"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374525"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)91648-C"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.18.7023"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1038\/363071a0"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1996.76.3.719"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1038\/366283a0"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.3.C1008"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1996.76.2.1297"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp017356"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(95)00138-7"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-0102(97)90638-6"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70182-3"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)90777-9"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.266.6.C1538"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/0300-9629(93)90274-8"},{"key":"B28","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1242\/jeb.187.1.19","volume":"187","author":"Okada Y.","year":"1994","journal-title":"J. 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