{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:11:29Z","timestamp":1760955089608,"version":"3.37.3"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":3368,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["Ab20069","AI-19884"],"award-info":[{"award-number":["Ab20069","AI-19884"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["Ab20069","AI-19884"],"award-info":[{"award-number":["Ab20069","AI-19884"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:p>Five stromal\u2010cell\u2010dependent lymphocyte clones are described that correspond to late pre\u2010B\nor early B\u2010cell stages of differentiation.They are useful for determining the molecular\nrequirements for pre\u2010B replication, for studying the stromal cells that supply those factors,\nand for delineating the final sequence of differentiation events as newly formed lymphocytes\nprepare to exit the bone marrow. The efficiency of lymphocyte growth at limiting dilution\nvaried substantially on different stromal\u2010cell clones and may reflect functional heterogeneity\nof stromal cells. Most lymphocyte clones were similar to uncloned lymphocytes from\nWhitlock\u2010Witte cultures in that they responded only transiently to interleukin\u20107 (IL\u20107) and\nthen died, unless maintained on a stromal\u2010cell clone. One unusual lymphocyte clone (2E8)\nwas propagated for more than 1 year in IL\u20107 alone and was selectively responsive to that\ncytokine. Most of the lymphocyte clones were not tumorigenic in immunodeficient mice.\nHowever, one pre\u2010B clone (1A9)\u2019grew autonomously in culture when held at high density,\nresponded to conditioned medium from a number of cell lines, and was tumorigenic.\nTumors derived from this clone were infiltrated by stromal cells and lymphocytes taken\nfrom the tumors\u2032 retained characteristics of the original clone. Ly\u20106 antigens were inducible\non 2E8 and 1A9 cells, but the lymphocytes were otherwise arrested in differentiation. The\n2E8 cells had rearranged and expressed <jats:italic>\u03ba<\/jats:italic> light\u2010chain genes but displayed them on the\nsurface along with surrogate light chains and <jats:italic>\u03bc<\/jats:italic> heavy chains. Thus, expression of authentic\nTight chain need not coincide with termination of surrogate light\u2010chain utilization in newly\nformed B cells. Several glycoproteins have recently been demonstrated to be associated with\nsurface immunoglobulin (Ig) on mature B\u2010lineage cells and plasma\u2010cell tumors. We now\nshow that one member of this family (approximately 33 kD) was associated with the <jats:italic>\u03bc<\/jats:italic>+surrogate light\u2010chain complex on the 1A9 pre\u2010B\u2010cell clone. When compared to mature B\nlymphomas, fewer bands coprecipitated with the surface\u2010labeled Ig isolated from pre\u2010B\u2010 and\nearly B\u2010cell lines, suggesting that components of the antigen receptor are sequentially\nacquired during development. The normal replication and differentiation of pre\u2010B cells is\nprobably regulated by complex interactions with multiple cytokines and matrix components\nof the marrow microenvironment. Cloned lymphocyte lines that are dependent on stromal\ncells should continue to be important tools for molecular definition of those interactions.<\/jats:p>","DOI":"10.1155\/1991\/79721","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T07:46:09Z","timestamp":1213170369000},"page":"149-161","source":"Crossref","is-referenced-by-count":17,"title":["Stromal\u2010Cell and Cytokine\u2010Dependent Lymphocyte Clones Which Span the Pre\u2010B\u2010 to B\u2010Cell Transition"],"prefix":"10.1155","volume":"1","author":[{"given":"Katsuhiko","family":"Ishihara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kay","family":"Medina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin-Ichi","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolynn","family":"Pietrangeli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony E.","family":"Namen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kensuke","family":"Miyake","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul W.","family":"Kincade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1990,10,12]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/1991\/079721.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/1991\/79721","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T20:38:54Z","timestamp":1723063134000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/1991\/79721"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,10,12]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1991,1]]}},"alternative-id":["10.1155\/1991\/79721"],"URL":"https:\/\/doi.org\/10.1155\/1991\/79721","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"type":"print","value":"2314-8861"},{"type":"electronic","value":"2314-7156"}],"subject":[],"published":{"date-parts":[[1990,10,12]]}}}