{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T08:24:45Z","timestamp":1759047885508,"version":"3.37.3"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":2922,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:p>To investigate the role of thymic myoid cells in the pathogenesis of myasthenia gravis\n(MG), mRNA of nonneoplastic thymuses from eight MG patients was analyzed by dot\nblot hybridization for the occurrence of acetylcholine receptor (AChR) subunit\ntranscripts, using the five AChR\u2010subunit cDNAs (alpha, beta, gamma, delta, and\nepsilon) as probes. Attention was particularly paid to the gamma\u2010 and epsilon\u2010subunit\ntranscripts that specify fetal\u2010 or adult\u2010type AChR. In all eight thymuses, transcripts of\nthe alpha\u2010, beta\u2010, gamma\u2010, and delta\u2010subunit genes were detected. Relative\nautoradiographic signal intensities correlated with the frequencies of thymic myoid cells\nas determined by immunostaining with anti\u2010AChR monoclonal antibodies. In only one\nof these thymuses were transcripts of the epsilon\u2010subunit gene detected in addition to\nthose of the other subunit genes. Four MG\u2010associated thymomas without myoid cells\nwere devoid of any AChR\u2010subunit mRNA. Our findings imply that fetal\u2010type AChR is\nexpressed in MG thymuses as a rule, whereas adult\u2010type AChR is coexpressed with it\nonly in a minority of cases. A similar pattern of cotranscription is known to occur at\ncertain stages of muscle development, and can be found in human rhabdomyosarcomas\nwith an intermediate stage of myogenesis. Because the serum autoantibodies of MG\npatients exhibit preferential reactivity with fetal AChRs, the presence of fetal AChRs in\nthe thymus provides circumstantial evidence for an active involvement of thymic myoid\ncells in the autoimmune process.<\/jats:p>","DOI":"10.1155\/1992\/40576","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T07:46:23Z","timestamp":1213170383000},"page":"69-75","source":"Crossref","is-referenced-by-count":22,"title":["Pathogenetic Significance of Fetal\u2010Type Acetylcholine Receptors onThymic Myoid Cells in Myasthenia Gravis"],"prefix":"10.1155","volume":"2","author":[{"given":"Kerstin I.","family":"Geuder","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Marx","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veit","family":"Witzemann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Berthold","family":"Schalke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Toyka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Kirchner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans-Konrad","family":"M\u00fcller-Hermelink","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1992,1]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/1992\/040576.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/1992\/40576","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T23:10:23Z","timestamp":1723072223000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/1992\/40576"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,1]]}},"alternative-id":["10.1155\/1992\/40576"],"URL":"https:\/\/doi.org\/10.1155\/1992\/40576","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"type":"print","value":"2314-8861"},{"type":"electronic","value":"2314-7156"}],"subject":[],"published":{"date-parts":[[1992,1]]}}}