{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T16:27:18Z","timestamp":1743438438822},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":2922,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:p>Introduction of a transgenic <jats:italic>\u03b1<\/jats:italic><jats:italic>\u00df<\/jats:italic> TCR (V<jats:italic>\u03b1<\/jats:italic><jats:sub>2<\/jats:sub>, V<jats:italic>\u00df<\/jats:italic><jats:sub>8.1<\/jats:sub>) specific for lymphocytic\nchoriomeningitis virus (LCMV), in the context of H\u20102D<jats:sup>b<\/jats:sup> into the genome of C57BL\/6\nmice, has many effects on the development and selection of T cells in both the thymus\nand the periphery. These mice produce increased numbers of CD4<jats:sup>\u2013<\/jats:sup>8<jats:sup>+<\/jats:sup> mature T cells, all\nof which express the transgenic TCR, and small numbers of CD4<jats:sup>\u2013<\/jats:sup>8<jats:sup>+<\/jats:sup>cells using\nendogenous TCRs are also produced. This study follows the intrathymic development\nof T cells in these TCR <jats:italic>\u03b1<\/jats:italic><jats:italic>\u00df<\/jats:italic> transgenic mice, in particular the earliest CD4<jats:sup>\u2013<\/jats:sup>8<jats:sup>\u2013<\/jats:sup> stages. As\nexpected, the transgenic TCR is expressed on the cell surface at an earlier developmental\nstage than endogenous TCRs in nontransgenic littermate controls. Of the three major\nsubsets expressing the heat\u2013stable antigen (HSA), only the most mature, the\nCD25<jats:sup>\u2013<\/jats:sup>CD44<jats:sup>\u2013<\/jats:sup> expresses the transgenic TCR, and the earlier CD25<jats:sup>\u2013<\/jats:sup>CD44<jats:sup>+<\/jats:sup> and\nCD25<jats:sup>+<\/jats:sup>CD44<jats:sup>\u2013<\/jats:sup> do not. Furthermore, in contrast to other TCR<jats:italic>\u03b1<\/jats:italic><jats:italic>\u00df<\/jats:italic>transgenic lines, TCR <jats:italic>\u03b3<\/jats:italic><jats:italic>\u03b4<\/jats:italic>\nlineage cells appear to develop normally.<\/jats:p>","DOI":"10.1155\/1992\/45150","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T07:46:23Z","timestamp":1213170383000},"page":"85-94","source":"Crossref","is-referenced-by-count":16,"title":["Analysis of Immature (CD4<sup>\u2013<\/sup>CD8<sup>\u2013<\/sup>) Thymic Subsets in T\u2010Cell Receptor<i>\u03b1<\/i><i>\u00df<\/i> Transgenic Mice"],"prefix":"10.1155","volume":"2","author":[{"given":"Anne","family":"Wilson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanspeter","family":"Pircher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela","family":"Ohashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. Robson","family":"Macdonald","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1992,1]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/1992\/045150.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/1992\/045150.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/1992\/45150","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T23:10:28Z","timestamp":1723072228000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/1992\/45150"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,1]]}},"alternative-id":["10.1155\/1992\/45150"],"URL":"https:\/\/doi.org\/10.1155\/1992\/45150","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"type":"print","value":"2314-8861"},{"type":"electronic","value":"2314-7156"}],"subject":[],"published":{"date-parts":[[1992,1]]}}}