{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T00:02:21Z","timestamp":1723075341376},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":2922,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Stichting Technische Wetenschappen","award":["PCT99.2105"],"award-info":[{"award-number":["PCT99.2105"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:p>T\u2010cell receptor (TCR) <jats:italic>\u00df<\/jats:italic>\u2010chain usage and expression of the CD3, CD4, and CD8\ndifferentiation antigens were analyzed in 14 spontaneous AKR lymphomas. Lymphoma\ncells massively infiltrated and\/or proliferated in the organs analyzed (thymus, spleen,\nand mesenteric lymph nodes), giving rise to a loss of organ structure. One lymphoma\noccurred only in the thymus, and failed to express CD3, CD4, and CD8. All other\nlymphomas expressed the CD3\/TCR complex. With respect to CD4 and CD8\nexpression, the lymphomas were either double\u2010negative (DN), double\u2010positive (DP), or\nsingle\u2010positive (SP). The frequency of DP (CD4<jats:sup>+<\/jats:sup>8<jats:sup>+<\/jats:sup>) lymphomas was low compared to the\nfrequency of DP thymocytes in a normal AKR thymus. A substantial heterogeneity was\nseen in the intensity of CD4 and CD8 expression among various lymphomas, which was\nindependent of the level of CD3 expression. Considering TCR V <jats:italic>\u00df<\/jats:italic> gene family usage, 2\nout of 14 lymphomas expressed V <jats:italic>\u00df<\/jats:italic>6. Normally, V <jats:italic>\u00df<\/jats:italic>6<jats:sup>+<\/jats:sup> thymocytes are deleted from the\nthymocyte pool at the immature DP stage of T\u2010cell development in AKR mice. These\ndata support the hypothesis that the lymphocytes in the immature DP stage of T\u2010cell\ndevelopment are susceptible to the induction of AKR lymphomagenesis. The presence\nof  V <jats:italic>\u00df<\/jats:italic>6<jats:sup>+<\/jats:sup> lymphoma cells indicates that the lymphomagenesis is accompanied by a\ndefective clonal deletion of cells expressing a possible autoreactive TCR.<\/jats:p>","DOI":"10.1155\/1992\/91527","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T07:46:23Z","timestamp":1213170383000},"page":"95-101","source":"Crossref","is-referenced-by-count":1,"title":["V<i>\u00df<\/i>Gene Family Usage in Spontaneous Lymphomas of AKR Mice:Evidence for Defective Clonal Deletion"],"prefix":"10.1155","volume":"2","author":[{"given":"Cees","family":"de Heer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"de Geus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henk-Jan","family":"Schuurma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henk","family":"Van Loveren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Rozing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1992,1]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/1992\/091527.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/1992\/91527","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T23:10:11Z","timestamp":1723072211000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/1992\/91527"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,1]]}},"alternative-id":["10.1155\/1992\/91527"],"URL":"https:\/\/doi.org\/10.1155\/1992\/91527","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"type":"print","value":"2314-8861"},{"type":"electronic","value":"2314-7156"}],"subject":[],"published":{"date-parts":[[1992,1]]}}}