{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T00:04:32Z","timestamp":1723075472137},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":2922,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Danish Research Council"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:p>Thymic epithelial cells (TEC) have been cultured for several months and\/or for 4 to 5\ntransfers in a growth factor\u2010defined serum\u2010free medium without concurrent growth of\nother cell types. The use of monoclonal antibodies and <jats:italic>\u03b1<\/jats:italic>MAM\u20106 indicated that the\nmajority of TEC were of medullary origin. The vast majority of cells were positive for\nLFA\u20103 and class I, and class II expression, was low or absent. Supernatants from the\ncultures were shown to contain IL\u20101<jats:italic>\u00df<\/jats:italic>, IL\u20106, and M\u2010CSF. Coculture of cloned\nsubpopulations of thymocytes and TEC showed effects of TEC and of secreted ILs on\nthymocyte proliferation. High percentages of TEC were able to bind DN, DP, or SP\nthymocyte populations, partly via CD2\u2010LFA\u20103 adhesion. Thus, it is possible to culture\nTEC without unknown serum factors and with maintenance of functional activities.<\/jats:p>","DOI":"10.1155\/1992\/95098","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T07:46:23Z","timestamp":1213170383000},"page":"111-121","source":"Crossref","is-referenced-by-count":14,"title":["Human Thymic Epithelial Cells in Serum\u2010Free Culture: Nature andEffects on Thymocyte Cell Lines"],"prefix":"10.1155","volume":"2","author":[{"given":"Carsten","family":"Ropke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jette","family":"Elbroend","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1992,1]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/1992\/095098.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/1992\/95098","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T23:10:26Z","timestamp":1723072226000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/1992\/95098"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,1]]}},"alternative-id":["10.1155\/1992\/95098"],"URL":"https:\/\/doi.org\/10.1155\/1992\/95098","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"type":"print","value":"2314-8861"},{"type":"electronic","value":"2314-7156"}],"subject":[],"published":{"date-parts":[[1992,1]]}}}