{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:49:26Z","timestamp":1770720566172,"version":"3.49.0"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":2556,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM-38306"],"award-info":[{"award-number":["GM-38306"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["IM-645"],"award-info":[{"award-number":["IM-645"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000048","name":"American Cancer Society","doi-asserted-by":"publisher","award":["GM-38306"],"award-info":[{"award-number":["GM-38306"]}],"id":[{"id":"10.13039\/100000048","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000048","name":"American Cancer Society","doi-asserted-by":"publisher","award":["IM-645"],"award-info":[{"award-number":["IM-645"]}],"id":[{"id":"10.13039\/100000048","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:p>The selective <jats:italic>in vitro<\/jats:italic> generation of rat, mouse, and human terminal deoxynucleotidyl\ntransferase\u2010positive (TdT<jats:sup>+<\/jats:sup> lymphoid cells in our long\u2010term xenogeneic bone marrow (BM)\nculture system is characterized by physical interaction between the developing lymphocytes\nand mouse BM\u2010adherent stromal cells and macrophages. In the present study, experiments\nin which micropor)us membrane culture inserts were inoculated with rat BM cells\ndemonstrated that although the generation of primitive B\u2010lineage lymphoid cells requires\nthe presence of a mouse BM feeder layer, cognitive recognition events are not necessary.\nSimilarly, cell\u2010free (and serum\u2010free) medium conditioned with mouse BM (but not thymus\nor spleen) adherent cells and stromal\u2010cell lines therefrom supported the proliferation of\nearly rat lymphoid cells in a dose\u2010dependent manner. Double immunofluorescence for\nincorporated bromo\u2010deoxyuridine (BrdU) and early B\u2010lineage markers of rat BM lymphoid\ncells maintained in culture inserts or conditioned medium (CM), and studies of their in vitro\nand <jats:italic>in vivo<\/jats:italic> developmental potentials, indicated that the lymphoproliferative response\nresulted from the selective stimulation of lymphoid stem and\/or progenitor cells. The most\nprimitive of these target cells had a HIS24<jats:sup>+<\/jats:sup> HIS50<jats:sup>-<\/jats:sup> TdT<jats:sup>-<\/jats:sup> c\u03bc<jats:sup>-<\/jats:sup> sIg<jats:sup>-<\/jats:sup>, pre\u2010pro\u2010B\u2010cell phenotype.\nWhereas this subset normally constitutes less than 2% of B\u2010lineage BM cells <jats:italic>in vivo<\/jats:italic>,\nit comprises more than 25% of total lymphoid cells <jats:italic>in vitro<\/jats:italic>. In addition, the number of TdT<jats:sup>+<\/jats:sup>\ncells, predominantly of the early pro\u2010B\u2010cell phenotype (HIS24<jats:sup>+<\/jats:sup> HIS50<jats:sup>-<\/jats:sup> TdT<jats:sup>-<\/jats:sup> c\u03bc<jats:sup>-<\/jats:sup> sIg<jats:sup>-<\/jats:sup>), was\nincreased approximately tenfold above input levels. Based on these and previous findings,\na schematic model is proposed for the developmental pathway of early B\u2010lineage cells in rat\nBM from the level of the committed (possibly common) lymphoid stem cell to that of the\npre\u2010B\u2010cell.<\/jats:p>","DOI":"10.1155\/1993\/13675","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T07:49:00Z","timestamp":1213170540000},"page":"181-195","source":"Crossref","is-referenced-by-count":2,"title":["A Selective Culture System for Generating TerminalDeoxynucleotidyl Transferase\u2010Positive Lymphoid Cells <i>In Vitro<\/i>. V. Detection of Stage\u2010Specific Pro\u2010B\u2010CellStimulating Activity in Medium Conditioned by Mouse Bone Marrow Stromal Cells"],"prefix":"10.1155","volume":"3","author":[{"given":"Sean D.","family":"Mckenna","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irving","family":"Goldschneider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1993,5,12]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/1993\/013675.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/1993\/13675","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T20:57:14Z","timestamp":1723064234000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/1993\/13675"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1993,1]]}},"alternative-id":["10.1155\/1993\/13675"],"URL":"https:\/\/doi.org\/10.1155\/1993\/13675","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"value":"2314-8861","type":"print"},{"value":"2314-7156","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,1]]}}}